Literature DB >> 10882680

Viability and functional activity of cryopreserved mononuclear cells.

A Weinberg1, L Zhang, D Brown, A Erice, B Polsky, M S Hirsch, S Owens, K Lamb.   

Abstract

Factors that influence viability and function of cryopreserved peripheral blood mononuclear cells (PBMC) were identified on 54 samples from 27 AIDS Clinical Trial Units. PBMC viability ranged from 1 to 96% with a median of 70%, was higher in laboratories with experienced staff, and was not significantly associated with CD4 cell number. Function of cryopreserved PBMC, measured by lymphocyte proliferation, was associated with viability. Preparations with viability greater than or equal to 70% had consistent proliferative responses and were suitable for functional analyses.

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Year:  2000        PMID： 10882680      PMCID： PMC95942          DOI： 10.1128/CDLI.7.4.714-716.2000

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


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2.  Functional heterogeneity among herpes simplex virus-specific human CD4+ T cells.

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