OBJECTIVE: To assess the efficacy of the cytoprotective B-amino acid taurine in prevention of skeletal muscle dysfunction secondary to ischaemia-reperfusion (IR) injury. DESIGN: Randomised controlled animal study. SETTING: Biomedical research laboratory, teaching hospital, Republic of Ireland. ANIMALS: 96 Sprague Dawley rats. INTERVENTIONS: Rats were randomised into three groups (n = 24/group): control (sham); ischaemia-reperfusion (untreated); and ischaemia-reperfusion + taurine. A further 24 rats were given taurine alone. The rat cremaster skeletal muscle model of four hours of ischaemia followed by reperfusion was used. Taurine 4%wt/vol was given in the animals' water throughout the experiment, beginning 48 hours before the ischaemia was initiated. OUTCOME MEASURES: 8 rats were killed from each group and muscle contractile function was assessed using electrical field stimulation in a tissue bath at 24 hrs, 48 hrs and 7 days. RESULTS: Ischaemia followed by 24 hours, 48 hours or 7 days of reperfusion resulted in significant reduction in both muscle twitch and tetanic contractile function (p < 0.05). This was reversed by taurine, which resulted in significant preservation of twitch and tetanic contractility at all time points including one week of reperfusion (p < 0.05). There was no difference in muscle function between muscle treated with taurine after ischaemia-reperfusion and control muscle, with the same operation but without ischaemia, from 48 hours onwards. CONCLUSIONS: These data show that pharmaceutical use of the endogenous amino acid taurine, unlike many-other agents, protects electrophysiological function in skeletal muscle against both early and late ischaemia-reperfusion injury.
OBJECTIVE: To assess the efficacy of the cytoprotective B-amino acid taurine in prevention of skeletal muscle dysfunction secondary to ischaemia-reperfusion (IR) injury. DESIGN: Randomised controlled animal study. SETTING: Biomedical research laboratory, teaching hospital, Republic of Ireland. ANIMALS: 96 Sprague Dawley rats. INTERVENTIONS:Rats were randomised into three groups (n = 24/group): control (sham); ischaemia-reperfusion (untreated); and ischaemia-reperfusion + taurine. A further 24 rats were given taurine alone. The rat cremaster skeletal muscle model of four hours of ischaemia followed by reperfusion was used. Taurine 4%wt/vol was given in the animals' water throughout the experiment, beginning 48 hours before the ischaemia was initiated. OUTCOME MEASURES: 8 rats were killed from each group and muscle contractile function was assessed using electrical field stimulation in a tissue bath at 24 hrs, 48 hrs and 7 days. RESULTS:Ischaemia followed by 24 hours, 48 hours or 7 days of reperfusion resulted in significant reduction in both muscle twitch and tetanic contractile function (p < 0.05). This was reversed by taurine, which resulted in significant preservation of twitch and tetanic contractility at all time points including one week of reperfusion (p < 0.05). There was no difference in muscle function between muscle treated with taurine after ischaemia-reperfusion and control muscle, with the same operation but without ischaemia, from 48 hours onwards. CONCLUSIONS: These data show that pharmaceutical use of the endogenous amino acid taurine, unlike many-other agents, protects electrophysiological function in skeletal muscle against both early and late ischaemia-reperfusion injury.
Authors: Stephen R Kearns; David E O'Briain; Katherine M Sheehan; Cathal Kelly; David Bouchier-Hayes Journal: Clin Orthop Relat Res Date: 2010-03-23 Impact factor: 4.176
Authors: Craig A Goodman; Deanna Horvath; Christos Stathis; Trevor Mori; Kevin Croft; Robyn M Murphy; Alan Hayes Journal: J Appl Physiol (1985) Date: 2009-05-07
Authors: Jessica R Terrill; Marisa N Duong; Rufus Turner; Caroline Le Guiner; Amber Boyatzis; Anthony J Kettle; Miranda D Grounds; Peter G Arthur Journal: Redox Biol Date: 2016-08-30 Impact factor: 11.799