Literature DB >> 10872319

72 residues of gal repressor fused to beta-galactosidase repress the gal operon of E. coli.

B von Wilcken-Bergmann1, M Koenen, H W Griesser, B Müller-Hill.   

Abstract

An active gene has been constructed which produces a chimera consisting of the N-terminal domain of the gal repressor and all but the first five residues of beta-galactosidase. Seventy two residues of gal repressor fused to beta-galactosidase as tetrameric core are sufficient to repress the gal operon in vivo and to bind to the gal operator in vitro.

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Year:  1983        PMID： 10872319      PMCID： PMC555271          DOI： 10.1002/j.1460-2075.1983.tb01580.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  21 in total

1.  PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.

Authors:  G R CRAVEN; E STEERS; C B ANFINSEN
Journal:  J Biol Chem       Date:  1965-06       Impact factor: 5.157

2.  Unusual location and function of the operator in the Escherichia coli galactose operon.

Authors:  R DiLauro; T Taniguchi; R Musso; B de Crombrugghe
Journal:  Nature       Date:  1979-06-07       Impact factor: 49.962

3.  Lac repressor. Specific proteolytic destruction of the NH 2 -terminal region and loss of the deoxyribonucleic acid-binding activity.

Authors:  T Platt; J G Files; K Weber
Journal:  J Biol Chem       Date:  1973-01-10       Impact factor: 5.157

4.  The amino-acid sequence of lac repressor.

Authors:  K Beyreuther; K Adler; N Geisler; A Klemm
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

5.  Relief of polarity in DNA-dependent cell-free synthesis of enzymes of the galactose operon of Escherichia coli.

Authors:  W Wetekam; K Staack; R Ehring
Journal:  Mol Gen Genet       Date:  1972

6.  How lac repressor binds to DNA.

Authors:  K Adler; K Beyreuther; E Fanning; N Geisler; B Gronenborn; A Klemm; B Müller-Hill; M Pfahl; A Schmitz
Journal:  Nature       Date:  1972-06-09       Impact factor: 49.962

7.  Construction, isolation and implications of repressor-galactosidase - beta-galactosidase hybrid molecules.

Authors:  J Kania; B Müller-Hill
Journal:  Eur J Biochem       Date:  1977-10-03

8.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

9.  Sequence of galR gene indicates a common evolutionary origin of lac and gal repressor in Escherichia coli.

Authors:  B von Wilcken-Bergmann; B Müller-Hill
Journal:  Proc Natl Acad Sci U S A       Date:  1982-04       Impact factor: 11.205

10.  Primary structure of the phage P22 repressor and its gene c2.

Authors:  R T Sauer; J Pan; P Hopper; K Hehir; J Brown; A R Poteete
Journal:  Biochemistry       Date:  1981-06-09       Impact factor: 3.162
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  5 in total

1.  Possible ideal lac operator: Escherichia coli lac operator-like sequences from eukaryotic genomes lack the central G X C pair.

Authors:  A Simons; D Tils; B von Wilcken-Bergmann; B Müller-Hill
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

2.  The upstream operator of the Escherichia coli galactose operon is sufficient for repression of transcription initiated at the cyclic AMP-stimulated promoter.

Authors:  G Kuhnke; A Krause; C Heibach; U Gieske; H J Fritz; R Ehring
Journal:  EMBO J       Date:  1986-01       Impact factor: 11.598

3.  Synthetic lac operator mediates repression through lac repressor when introduced upstream and downstream from lac promoter.

Authors:  M Besse; B von Wilcken-Bergmann; B Müller-Hill
Journal:  EMBO J       Date:  1986-06       Impact factor: 11.598

4.  Leaf-specific thionins of barley-a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defence mechanism of plants.

Authors:  H Bohlmann; S Clausen; S Behnke; H Giese; C Hiller; U Reimann-Philipp; G Schrader; V Barkholt; K Apel
Journal:  EMBO J       Date:  1988-06       Impact factor: 11.598

5.  Characterization of two mutations in the Escherichia coli galE gene inactivating the second galactose operator and comparative studies of repressor binding.

Authors:  H J Fritz; H Bicknäse; B Gleumes; C Heibach; S Rosahl; R Ehring
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
  5 in total

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