Literature DB >> 108667

Antibody to B. subtilis DNA polymerase III: use in enzyme purification and examination of homology among replication-specific DNA polymerases.

M H Barnes, N C Brown.   

Abstract

Bacillus subtilis DNA polymerase III (pol III), an arylhydrazinopyrimidine-sensitive, replication-specific enzyme, was used to generate a non-precipitating rabbit antibody which specifically inhibited pol III activity in vitro. The antibody was used to examine structural relationships among several DNA polymerases, and it was linked covalently to agarose; the antibody:agarose was employed to develop a rapid, selective method of purification of catalytically active B. subtilis pol III.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 108667      PMCID： PMC327764          DOI： 10.1093/nar/6.3.1203

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  20 in total

1.  Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.

Authors:  S W Kessler
Journal:  J Immunol       Date:  1975-12       Impact factor: 5.422

Review 2.  DNA replication.

Authors:  M L Gefter
Journal:  Annu Rev Biochem       Date:  1975       Impact factor: 23.643

3.  REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

Authors:  C Anagnostopoulos; J Spizizen
Journal:  J Bacteriol       Date:  1961-05       Impact factor: 3.490

4.  DNA polymerase III holoenzyme of Escherichia coli. Purification and resolution into subunits.

Authors:  C McHenry; A Kornberg
Journal:  J Biol Chem       Date:  1977-09-25       Impact factor: 5.157

5.  A rapid, sensitive, and specific method for the determination of protein in dilute solution.

Authors:  W Schaffner; C Weissmann
Journal:  Anal Biochem       Date:  1973-12       Impact factor: 3.365

6.  Human leukaemic cells contain reverse transcriptase associated with a high molecular weight virus-related RNA.

Authors:  W Baxt; R Hehlmann; S Spiegelman
Journal:  Nat New Biol       Date:  1972-11-15

7.  Immunoaffinity chromatography of proteins.

Authors:  D M Livingston
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

8.  Effect of 6-(p-hydroxyphenyl)-azouracil on B. subtilis DNA polymerases.

Authors:  G W Bazill; J D Gross
Journal:  Nat New Biol       Date:  1972-11-15

9.  Cell membrane antigen isolation with the staphylococcal protein A-antibody adsorbent.

Authors:  S W Kessler
Journal:  J Immunol       Date:  1976-11       Impact factor: 5.422

10.  Actin antibody: the specific visualization of actin filaments in non-muscle cells.

Authors:  E Lazarides; K Weber
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205
View more
  13 in total

1.  Hybrid antibacterials. DNA polymerase-topoisomerase inhibitors.

Authors:  Chengxin Zhi; Zheng-Yu Long; Andrzej Manikowski; Jeanne Comstock; Wei-Chu Xu; Neal C Brown; Paul M Tarantino; Karsten A Holm; Edward J Dix; George E Wright; Marjorie H Barnes; Michelle M Butler; Kimberly A Foster; William A LaMarr; Benoit Bachand; Richard Bethell; Caroline Cadilhac; Sylvie Charron; Serge Lamothe; Irina Motorina; Richard Storer
Journal:  J Med Chem       Date:  2006-02-23       Impact factor: 7.446

2.  Butylanilinouracil: a selective inhibitor of HeLa cell DNA synthesis and HeLa cell DNA polymerase alpha.

Authors:  G E Wright; E F Baril; N C Brown
Journal:  Nucleic Acids Res       Date:  1980-01-11       Impact factor: 16.971

3.  Inhibitors of DNA polymerase III as novel antimicrobial agents against gram-positive eubacteria.

Authors:  P M Tarantino; C Zhi; G E Wright; N C Brown
Journal:  Antimicrob Agents Chemother       Date:  1999-08       Impact factor: 5.191

4.  Low frequencies of resistance among Staphylococcus and Enterococcus species to the bactericidal DNA polymerase inhibitor N(3)-hydroxybutyl 6-(3'-ethyl-4'-methylanilino) uracil.

Authors:  Michelle M Butler; Donna J Skow; Ryan O Stephenson; Patrick T Lyden; William A LaMarr; Kimberly A Foster
Journal:  Antimicrob Agents Chemother       Date:  2002-12       Impact factor: 5.191

5.  Development of novel inhibitor probes of DNA polymerase III based on dGTP analogs of the HPUra type: base, nucleoside and nucleotide derivatives of N2-(3,4-dichlorobenzyl)guanine.

Authors:  M M Butler; L W Dudycz; N N Khan; G E Wright; N C Brown
Journal:  Nucleic Acids Res       Date:  1990-12-25       Impact factor: 16.971

6.  Antibacterial activity and mechanism of action of a novel anilinouracil-fluoroquinolone hybrid compound.

Authors:  Michelle M Butler; William A Lamarr; Kimberly A Foster; Marjorie H Barnes; Donna J Skow; Patrick T Lyden; Lauren M Kustigian; Chengxin Zhi; Neal C Brown; George E Wright; Terry L Bowlin
Journal:  Antimicrob Agents Chemother       Date:  2006-10-30       Impact factor: 5.191

7.  Clostridium difficile DNA polymerase IIIC: basis for activity of antibacterial compounds.

Authors:  Andrea Torti; Andrea Lossani; Lida Savi; Federico Focher; George Edward Wright; Neal Curtis Brown; Wei-Chu Xu
Journal:  Curr Enzym Inhib       Date:  2011-10

8.  Butylphenyl dGTP: a selective and potent inhibitor of mammalian DNA polymerase alpha.

Authors:  N N Khan; G E Wright; L W Dudycz; N C Brown
Journal:  Nucleic Acids Res       Date:  1984-04-25       Impact factor: 16.971

9.  Design and characterization of N2-arylaminopurines which selectively inhibit replicative DNA synthesis and replication-specific DNA polymerases: guanine derivatives active on mammalian DNA polymerase alpha and bacterial DNA polymerase III.

Authors:  G E Wright; E F Baril; V M Brown; N C Brown
Journal:  Nucleic Acids Res       Date:  1982-07-24       Impact factor: 16.971

10.  Cloning and characterization of the polC region of Bacillus subtilis.

Authors:  R W Ott; M H Barnes; N C Brown; A T Ganesan
Journal:  J Bacteriol       Date:  1986-03       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.