Literature DB >> 1086615

Changes of activity of alkaline and acid phosphatase in the rabbit eye in the early phase of alkaline and acid injury.

A Bolková, J Obenberger.   

Abstract

Rabbit corneas were burned either with 1.0 N sodium hydroxide or 1.0 N hydrochloric acid. Enzyme activities of alkaline and acid phosphatases were examined spectrophotometrically in the homogenates of cornea, iris, aqueous humor and vitreous body. On the 3rd day after alkaline as well as acid burn, a significant decrease of both enzyme activities was produced as compared with untreated animals. A more pronounced change was found in the case of alkaline injuries. With both kinds of caustic agents the decrease of acid phosphatase activity was more striking than that of the alkaline phosphatase. Advantages and shortcomings of biochemical and histochemical enzymatic determinations in experimental ocular inflammations are briefly discussed.

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Year:  1976        PMID： 1086615     DOI： 10.1007/bf01028541

Source DB:  PubMed          Journal:  Albrecht Von Graefes Arch Klin Exp Ophthalmol        ISSN： 0065-6100


  7 in total

1.  Ring-shaped alkali burns of the rabbit cornea. II. Early changes of the corneal stroma.

Authors:  F Vrabec; J Obenberger
Journal:  Albrecht Von Graefes Arch Klin Exp Ophthalmol       Date:  1976-02-04

2.  Ring-shaped alkali burns of the rabbit cornea. Epithelial and endothelial changes.

Authors:  F Vrabec; J Obenberger; J Vrabec
Journal:  Albrecht Von Graefes Arch Klin Exp Ophthalmol       Date:  1975-12-04

3.  Distribution of 24NaCl in tissues of alkali- and acid-burned rabbit eyes.

Authors:  J Obenberger; A Babický
Journal:  Exp Eye Res       Date:  1975-03       Impact factor: 3.467

4.  A method for the rapid determination of alkaline phosphates with five cubic millimeters of serum.

Authors:  O A BESSEY; O H LOWRY; M J BROCK
Journal:  J Biol Chem       Date:  1946-07       Impact factor: 5.157

5.  Alkali burns of the rabbit cornea. I. A histochemical study of beta-glucuronidase, beta-galactosidase and N-acetyl-beta-D-glucosaminidase.

Authors:  J Cejková; Z Lojda; J Obenberger; E Havránková
Journal:  Histochemistry       Date:  1975-09-07

6.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

7.  [Alkaline phosphatase and inorganic phosphorus content in the cornea after a chemical burn].

Authors:  P S Kaplunovich; I I Eremina; M B Korina; G N Skriabina
Journal:  Vestn Oftalmol       Date:  1973 May-Jun
  7 in total
  10 in total

1.  Biochemical study on some acid hydrolases in the normal rabbit cornea.

Authors:  A Bolková
Journal:  Albrecht Von Graefes Arch Klin Exp Ophthalmol       Date:  1977-07-08

2.  The influence of various toxic effects on the cornea and changes in corneal light transmission.

Authors:  Cestmír Cejka; Taras Ardan; Jakub Sirc; Jiří Michálek; Blanka Brůnová; Jitka Cejková
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  2010-07-02       Impact factor: 3.117

3.  Acid hydrolases in the bovine corneal epithelium.

Authors:  T Shiono; S Hayasaka; K Mizuno
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  1986       Impact factor: 3.117

4.  A study on alkaline phosphatase in cornea of various animals with special regard to keratocytes.

Authors:  J Cejková; A Bolková
Journal:  Albrecht Von Graefes Arch Klin Exp Ophthalmol       Date:  1977-11-25

5.  Relationship between various concentrations of NaOH and metabolic effects in experimentally burned rabbit cornea. A biochemical and histochemical study.

Authors:  A Bolková; J Cejková
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  1984       Impact factor: 3.117

6.  Changes in alkaline and acid phosphatases of the rabbit cornea following experimental exposure to ethanol and acetone: a biochemical and histochemical study.

Authors:  A Bolková; J Cejková
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  1983       Impact factor: 3.117

7.  The alkali burned cornea: electron microscopical, enzyme histochemical, and biochemical observations.

Authors:  T Pahlitzsch; P Sinha
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  1985       Impact factor: 3.117

8.  Hydrolases of anterior segment tissues in the normal human, pig and rat eye: a comparative study.

Authors:  S E Coupland; P L Penfold; F A Billson
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  1994-03       Impact factor: 3.117

9.  Histochemical survey of the anterior segment of the normal human foetal and adult eye.

Authors:  S E Coupland; P L Penfold; F A Billson
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  1993-09       Impact factor: 3.117

10.  Enzyme activities in the rabbit cornea during immunogenic keratitis.

Authors:  N J van Haeringen; A Bolkova; J A Oosterhuis; J L van Delft
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  1986       Impact factor: 3.117
  10 in total

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