| Literature DB >> 10863037 |
J Nakauchi1, H Matsuo, D K Kim, A Goto, A Chairoungdua, S H Cha, J Inatomi, Y Shiokawa, K Yamaguchi, I Saito, H Endou, Y Kanai.
Abstract
We isolated a cDNA for the human homologue of system asc transporter Asc-1 from human brain. The encoded protein designated as hAsc-1 (human Asc-1) exhibited 91 % sequence identity to mouse Asc-1. Consistent with mouse Asc-1, hAsc-1 required 4F2 heavy chain for its functional expression in Xenopus oocytes. hAsc-1 exhibited the properties of amino acid transport system asc which transports small neutral amino acids in a Na(+)-independent manner. hAsc-1 transported D-serine at high affinity with a K(m) value of 22.8 microM. In brain, 2.0 kb mRNA was highly expressed. hAsc-1 gene was mapped to human chromosome 19, region q12-q13.1. Because of the high-affinity transport with the K(m) value close to the physiological concentration of D-serine, together with the high levels of expression in brain, hAsc-1 is proposed to play significant roles in the D-serine mobilization in brain.Entities:
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Year: 2000 PMID： 10863037 DOI： 10.1016/s0304-3940(00)01169-1
Source DB: PubMed Journal: Neurosci Lett ISSN： 0304-3940 Impact factor: 3.046