Literature DB >> 1085784

Single step separation of human T and B cells using AET treated srbc rosettes.

A Saxon, J Feldhaus, R A Robins.   

Abstract

A technique for the single step separation of human thymus derived (T) and Bursa equivalent (B) lymphocytes was developed. Density separation of B lymphocytes and T lymphocyte-sheep red blood cell (SRBC) rosettes on Ficoll-Hypaque was modified by pretreatment of the SRBC with 2 aminoethylisothiouronium bromide hydrobromide. This modification yielded significantly purer populations of T and B cells. Up to 15 X 10(7) were separated without increasing contamination allowing for the recovery of B lymphocytes as well as T lymphocytes in sufficient numbers to use in functional assays.

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Year:  1976        PMID： 1085784     DOI： 10.1016/0022-1759(76)90050-8

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  98 in total

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8.  Lamivudine treatment can restore T cell responsiveness in chronic hepatitis B.

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