Literature DB >> 10854858

Thrombin stimulation of platelets causes an increase in phosphatidylinositol 5-phosphate revealed by mass assay.

J B Morris1, K A Hinchliffe, A Ciruela, A J Letcher, R F Irvine.   

Abstract

Phosphatidylinositol 5-phosphate (PtdIns5P), a novel inositol lipid, has been shown to be the major substrate for the type II PtdInsP kinases (PIPkins) ¿Rameh et al. (1997) Nature 390, 192-196. A PtdInsP fraction was prepared from cell extracts by neomycin chromatography, using a protocol devised to eliminate the interaction of acidic solvents with plasticware, since this was found to inhibit the enzyme. The PtdIns5P in this fraction was measured by incubating with ¿gamma-(32)PATP and recombinant PIPkin IIalpha, and quantifying the radiolabelled PtdInsP(2) formed. This assay was used on platelets to show that during 10 min stimulation with thrombin, the mass level of PtdIns5P increases, implying the existence of an agonist-stimulated synthetic mechanism.

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Year:  2000        PMID： 10854858     DOI： 10.1016/s0014-5793(00)01625-2

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  35 in total

1.  A novel HPLC-based approach makes possible the spatial characterization of cellular PtdIns5P and other phosphoinositides.

Authors:  Deborah Sarkes; Lucia E Rameh
Journal:  Biochem J       Date:  2010-05-27       Impact factor: 3.857

2.  New methods for capturing the mystery lipid, PtdIns5P.

Authors:  Jonathan M Backer
Journal:  Biochem J       Date:  2010-05-27       Impact factor: 3.857

Review 3.  PIKfyve: Partners, significance, debates and paradoxes.

Authors:  Assia Shisheva
Journal:  Cell Biol Int       Date:  2008-01-25       Impact factor: 3.612

4.  Irreversible platelet activation requires protease-activated receptor 1-mediated signaling to phosphatidylinositol phosphates.

Authors:  Michael Holinstat; Anita M Preininger; Stephen B Milne; W James Hudson; H Alex Brown; Heidi E Hamm
Journal:  Mol Pharmacol       Date:  2009-05-29       Impact factor: 4.436

5.  The identification and characterization of two phosphatidylinositol-4,5-bisphosphate 4-phosphatases.

Authors:  Alexander Ungewickell; Christopher Hugge; Marina Kisseleva; Shao-Chun Chang; Jun Zou; Yucheng Feng; Edouard E Galyov; Monita Wilson; Philip W Majerus
Journal:  Proc Natl Acad Sci U S A       Date:  2005-12-19       Impact factor: 11.205

6.  The nucleophosmin-anaplastic lymphoma kinase oncogene interacts, activates, and uses the kinase PIKfyve to increase invasiveness.

Authors:  Sophie Dupuis-Coronas; Frédéric Lagarrigue; Damien Ramel; Gaëtan Chicanne; Estelle Saland; Frédérique Gaits-Iacovoni; Bernard Payrastre; Hélène Tronchère
Journal:  J Biol Chem       Date:  2011-07-07       Impact factor: 5.157

7.  Phosphatidylinositol 5-phosphate 4-kinase (PIP4K) regulates TOR signaling and cell growth during Drosophila development.

Authors:  Amit Gupta; Sarah Toscano; Deepti Trivedi; David R Jones; Swarna Mathre; Jonathan H Clarke; Nullin Divecha; Padinjat Raghu
Journal:  Proc Natl Acad Sci U S A       Date:  2013-03-25       Impact factor: 11.205

8.  Distribution and neuronal expression of phosphatidylinositol phosphate kinase IIgamma in the mouse brain.

Authors:  Jonathan H Clarke; Piers C Emson; Robin F Irvine
Journal:  J Comp Neurol       Date:  2009-11-20       Impact factor: 3.215

9.  Production of phosphatidylinositol 5-phosphate via PIKfyve and MTMR3 regulates cell migration.

Authors:  Angela Oppelt; Viola H Lobert; Kaisa Haglund; Ashley M Mackey; Lucia E Rameh; Knut Liestøl; Kay Oliver Schink; Nina Marie Pedersen; Eva M Wenzel; Ellen M Haugsten; Andreas Brech; Tor Erik Rusten; Harald Stenmark; Jørgen Wesche
Journal:  EMBO Rep       Date:  2012-11-16       Impact factor: 8.807

Review 10.  Localization, regulation and function of type II phosphatidylinositol 5-phosphate 4-kinases.

Authors:  Jonathan H Clarke; Michael Wang; Robin F Irvine
Journal:  Adv Enzyme Regul       Date:  2009-11-06
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