Continuous culturing of murine splenic B-lumphocytes: synthesis and surface deposition of IgM and putative IgD molecules.

A method for the continuous culturing of mouse splenic lymphocytes is described. The method utilizes rat-tail collagen-coated glass or plastic surfaces which enhance the adherence and proliferation of B-lymphocytes. The cultured cells are shown to synthesize IgM and putative IgD molecules which are deposited on the cell membrane. The majority of the cells possessed Fc receptors and C' receptors, while none of them seemed to possess theta-antigen. It is concluded that these cells are functionally active B-lymphoid cells.