X Y Zhong1, W Holzgreve, S Hahn. 1. Department of Obstetrics and Gynaecology, University of Basel, Switzerland.
Abstract
OBJECTIVE: To test the sensitivity, specificity and reproducibility using fetal DNA obtained from plasma of pregnant women by polymerase chain reaction for the simultaneous detection of both fetal sex and Rhesus D genotype. METHOD: Blood samples were obtained from 22 Rhesus D negative pregnant women about to undergo an invasive procedure. DNA was extracted from the plasma fraction and analysed by a multiplex nested polymerase chain reaction using Y chromosome-and Rhesus D-specific primers. The results of this experimental procedure were compared with those obtained from the analysis performed on material gained by the invasive procedure. RESULTS: The sensitivity of the plasma polymerase chain reaction-based method was surprisingly high, with both fetal genotypes being correctly determined in almost 100% of the cases examined. In only one instance was a false positive result for the detection of Rhesus D recorded, which on subsequent analysis was negative. CONCLUSIONS: The ease and rapidity with which the plasma polymerase chain reaction-based method can be performed makes it a promising method for the analysis of multiple fetal loci, such as Rhesus D and sex.
OBJECTIVE: To test the sensitivity, specificity and reproducibility using fetal DNA obtained from plasma of pregnant women by polymerase chain reaction for the simultaneous detection of both fetal sex and Rhesus D genotype. METHOD: Blood samples were obtained from 22 Rhesus D negative pregnant women about to undergo an invasive procedure. DNA was extracted from the plasma fraction and analysed by a multiplex nested polymerase chain reaction using Y chromosome-and Rhesus D-specific primers. The results of this experimental procedure were compared with those obtained from the analysis performed on material gained by the invasive procedure. RESULTS: The sensitivity of the plasma polymerase chain reaction-based method was surprisingly high, with both fetal genotypes being correctly determined in almost 100% of the cases examined. In only one instance was a false positive result for the detection of Rhesus D recorded, which on subsequent analysis was negative. CONCLUSIONS: The ease and rapidity with which the plasma polymerase chain reaction-based method can be performed makes it a promising method for the analysis of multiple fetal loci, such as Rhesus D and sex.
Authors: Giovanna Vona; Christophe Béroud; Alexandra Benachi; Alice Quenette; Jean Paul Bonnefont; Serge Romana; Yves Dumez; Bernard Lacour; Patrizia Paterlini-Bréchot Journal: Am J Pathol Date: 2002-01 Impact factor: 4.307
Authors: Ilona Hromadnikova; Bela Houbova; Dana Hridelova; Sona Voslarova; Pavel Calda; Katerina Nekolarova; Josef Kofer; David Stejskal; Jindrich Doucha; Ondrej Cinek; Jan Vavrirec Journal: BMC Pregnancy Childbirth Date: 2002-05-28 Impact factor: 3.007