Literature DB >> 1084381

Determination of alpha1-antitrypsin phenotypes by isoelectric focusing in polyacrylamide gels.

F Kueppers.   

Abstract

A method for alpha1-antitrypsin phenotyping is described that uses isoelectric focusing in a pH gradient 3.5 to 5 with polyacrylamide as supporting medium. The method is fast, reproducible, and reliable and offers some advantages over the currently used starch-gel electrophoresis.

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Year:  1976        PMID： 1084381

Source DB:  PubMed          Journal:  J Lab Clin Med        ISSN： 0022-2143


  20 in total

1.  Simultaneous phenotyping and quantification of α-1-antitrypsin by liquid chromatography-tandem mass spectrometry.

Authors:  Yuhong Chen; Melissa R Snyder; Yi Zhu; Linda J Tostrud; Linda M Benson; Jerry A Katzmann; H Robert Bergen
Journal:  Clin Chem       Date:  2011-06-02       Impact factor: 8.327

2.  Biochemical reaction of alpha 1 antitrypsin during the substitution therapy of patients with homozygote PI-ZZ deficit.

Authors:  A Gillissen; E W Schmidt; B Rasche; W T Ulmer
Journal:  Klin Wochenschr       Date:  1989-03-15

3.  Alpha1-antitrypsin: further genetic heterogeneity revealed by isoelectric focusing.

Authors:  F Kueppers; M J Christopherson
Journal:  Am J Hum Genet       Date:  1978-07       Impact factor: 11.025

4.  Alpha-1-antitrypsin phenotypes in Lyon, France.

Authors:  P Arnaud; C Chapuis-Cellier; P Vittoz; R Creyssel
Journal:  Hum Genet       Date:  1977-11-02       Impact factor: 4.132

5.  Mechanism of inhibition of porcine elastase by human alpha-1-antitrypsin.

Authors:  H L James; A B Cohen
Journal:  J Clin Invest       Date:  1978-12       Impact factor: 14.808

6.  Frequency and significance of phenotypes for alpha1-antitrypsin deficiency in type 1 autoimmune hepatitis.

Authors:  A J Czaja
Journal:  Dig Dis Sci       Date:  1998-08       Impact factor: 3.199

7.  [Clinical-functional emphysema diagnostics and immunological correlations (author's transl)].

Authors:  J Kowalski; B Rasche; A A Bulgalho de Almeida; K Hochstrasser; W T Ulmer
Journal:  Klin Wochenschr       Date:  1979-05-16

8.  Pulmonary emphysema associated with the FZ alpha 1-antitrypsin phenotype.

Authors:  D W Cockcroft; R K Tennent; S L Horne
Journal:  Can Med Assoc J       Date:  1981-03-15       Impact factor: 8.262

9.  Alpha 1-antitrypsin: apparent molecular weight heterogeneity shown by two-dimensional electrophoresis.

Authors:  W T Brown
Journal:  Am J Hum Genet       Date:  1982-03       Impact factor: 11.025

10.  Glomerulonephritis as late manifestation of severe alpha 1-antitrypsin deficiency.

Authors:  R E Stauber; J H Horina; M Trauner; G J Krejs; M Ratschek; M Klimpfinger
Journal:  Clin Investig       Date:  1994-05
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