Literature DB >> 1084002

[Electrophoretic mobility of human lymphocytes purified by nylon columns and spontaneous rosettes].

P Chollet, J Chassagne, D Guérin, B Sauvezie, J M Bidet, G Bétail, R Plagne.   

Abstract

Cell electrophoresis enables the separation of the lymphocytes in normal human blood into two principal groups, as a function of their migration speed in relation to 1 mum.sec -1..v-1.cm. In 42 healthy adults, 19,9 % of the lymphocytes have a slower migration, and 80,1 % a faster migration than the reference speed. Two known methods are used for the selection of the lymphocytic populations : spontaneous rosetting with sheep red blood cells, a property of the T lymphocytes, and the adherence to nylon wool columns, which is dominant in the case of B lymphocytes. The cells which do not form spontaneous rosettes, and the cells adhering to nylon wool columns have above all a slow migration. On the contrary, cells which do not adhere to nylon columns have a fast migration. These arguments are in favour of the T nature of the rapid migrating lymphocytes, and of the B nature of the slow migrating lymphocytes.

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Year:  1976        PMID: 1084002

Source DB:  PubMed          Journal:  Rev Can Biol        ISSN: 0035-0915


  1 in total

1.  Electrokinetic study of the reactions of peritoneal macrophages and eosinophils with IgG immune complexes.

Authors:  H R Petty; R L Folger; B R Ware
Journal:  Cell Biophys       Date:  1979-03
  1 in total

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