BACKGROUND/AIM: The aim of this study is to improve the obstetrician-based cord blood collection system and an efficient recovery of CD34+ haematopoietic progenitor stem cells. METHODS: CD34+ cells were purified from total blood using a positive selection enrichment method, called Mini-Macs. RESULTS: The final yield of CD34+ cells we obtained was 10(4) cells/ml, with a CD34+ purity of 99%. CONCLUSION: Our results confirm that, by using this method, it is possible to get a significant stem cell number, thus improving transplanting both peripheral stem cells and umbilical cord ones. Copyright 2000 S. Karger AG, Basel
BACKGROUND/AIM: The aim of this study is to improve the obstetrician-based cord blood collection system and an efficient recovery of CD34+ haematopoietic progenitor stem cells. METHODS:CD34+ cells were purified from total blood using a positive selection enrichment method, called Mini-Macs. RESULTS: The final yield of CD34+ cells we obtained was 10(4) cells/ml, with a CD34+ purity of 99%. CONCLUSION: Our results confirm that, by using this method, it is possible to get a significant stem cell number, thus improving transplanting both peripheral stem cells and umbilical cord ones. Copyright 2000 S. Karger AG, Basel
Authors: Svitlana Garbuzova-Davis; Maria C O Rodrigues; Santhia Mirtyl; Shanna Turner; Shazia Mitha; Jasmine Sodhi; Subatha Suthakaran; David J Eve; Cyndy D Sanberg; Nicole Kuzmin-Nichols; Paul R Sanberg Journal: PLoS One Date: 2012-02-03 Impact factor: 3.240
Authors: Jose N Tolosa; Dong-Hyuk Park; David J Eve; Stephen K Klasko; Cesario V Borlongan; Paul R Sanberg Journal: J Cell Mol Med Date: 2010-02-05 Impact factor: 5.310
Authors: Svitlana Garbuzova-Davis; Cyndy Davis Sanberg; Nicole Kuzmin-Nichols; Alison E Willing; Carmelina Gemma; Paula C Bickford; Christina Miller; Robert Rossi; Paul R Sanberg Journal: PLoS One Date: 2008-06-25 Impact factor: 3.240