Culture of organized cell communities.

Cells cultured in vitro will tend to retain their differentiated phenotype under conditions that resemble their natural in vivo environment, for example, when cultured on polymer scaffolds in tissue culture bioreactors. In this chapter, we define organized cell communities as three-dimensional in vitro grown cell-polymer constructs that display important structural and functional features of the natural tissue. We review representative studies in which the research goal was to culture organized cell communities resembling cartilage, bone, skeletal muscle or cardiac-like tissue. These constructs can potentially serve as tissue equivalents for in vivo transplantation or as a model system for the in vitro testing of cell and tissue-level responses to molecular, mechanical or genetic manipulations.