OBJECTIVES: The objectives of this study were to examine the frequency of lymph node micrometastases detected by keratin immunohistochemistry and their relationship with survival behaviour. METHODS: A total of 133 consecutive patients staged as Duke's B, who had curative resection for colorectal cancer (CRC), comprised the study population. Patients who had died of a non-CRC-related cause or who became lost to follow-up were excluded, resulting in an amended population of 100. Study end-points were defined as disease-free survival of 5 years or CRC-related death. Paraffin-embedded lymph node sections were stained with a commercial cytokeratin antibody using a standard avidin-biotin technique. RESULTS: One quarter of subjects had micrometastases. Fifty-six per cent of subjects with positive lymph nodes had an adverse outcome, compared with 11% of subjects with negative nodes. A highly significant association was found between lymph node cytokeratin expression and mortality in both the univariate (log rank P = 0.0001) and multivariate (Cox proportional hazards P = 0.0123) analysis. CONCLUSIONS: Lymph node micrometastases detected by this inexpensive and simple technique are significantly associated with mortality in Duke's B CRC. This technique may be used to select patients for adjuvant chemotherapy.
OBJECTIVES: The objectives of this study were to examine the frequency of lymph node micrometastases detected by keratin immunohistochemistry and their relationship with survival behaviour. METHODS: A total of 133 consecutive patients staged as Duke's B, who had curative resection for colorectal cancer (CRC), comprised the study population. Patients who had died of a non-CRC-related cause or who became lost to follow-up were excluded, resulting in an amended population of 100. Study end-points were defined as disease-free survival of 5 years or CRC-related death. Paraffin-embedded lymph node sections were stained with a commercial cytokeratin antibody using a standard avidin-biotin technique. RESULTS: One quarter of subjects had micrometastases. Fifty-six per cent of subjects with positive lymph nodes had an adverse outcome, compared with 11% of subjects with negative nodes. A highly significant association was found between lymph node cytokeratin expression and mortality in both the univariate (log rank P = 0.0001) and multivariate (Cox proportional hazards P = 0.0123) analysis. CONCLUSIONS: Lymph node micrometastases detected by this inexpensive and simple technique are significantly associated with mortality in Duke's B CRC. This technique may be used to select patients for adjuvant chemotherapy.
Authors: Masaki Hata; Junji Machi; Jonathan Mamou; Eugene T Yanagihara; Emi Saegusa-Beecroft; Gregory K Kobayashi; Clifford C M Wong; Conway Fung; Ernest J Feleppa; Kazuhiro Sakamoto Journal: Pathol Oncol Res Date: 2011-04-15 Impact factor: 3.201
Authors: José A García-Sáenz; María C Sáenz; Lucía González; Pedro Pérez-Segura; Javier Puente; Sara López-Tarruella; Javier Sastre; Antonio Casado; José G López-Asenjo; Eduardo Díaz-Rubio Journal: Clin Transl Oncol Date: 2006-09 Impact factor: 3.405
Authors: Robert Rosenberg; Jan Friederichs; Ralf Gertler; Axel Hoos; James Mueller; Jorg Nahrig; Hjalmar Nekarda; Joerg-Ruediger Siewert Journal: Int J Colorectal Dis Date: 2004-01-10 Impact factor: 2.571
Authors: Khaled M Madbouly; Anthony J Senagore; Abir Mukerjee; Conor P Delaney; Jason Connor; Victor W Fazio Journal: Int J Colorectal Dis Date: 2006-03-10 Impact factor: 2.571