Advances in diagnosis of tuberculosis.

The diagnosis of childhood tuberculosis is acknowledged to be an imprecise process since bacteriological confirmation is available in only 30-40% of cases. Newer developments in diagnosis of tuberculosis include use of fluorescent stains for smears, newer systems for radiometric detection of mycobacteria, rapid sensitivity testing using firefly bioluminescence, liquid chromatographic analysis of mycolic acids, immunodiagnostics for M. tuberculosis specific antigens and the impact of molecular diagnostics with amplification methods. The search for simple, reliable test for early stages of the disease (in particular TB meningitis) still continues.