J R Harris1, J Markl. 1. Institute of Zoology, University of Mainz, Germany. rharris@uzomai.biologie.uni-mainz.de
Abstract
OBJECTIVES: In this short review we present a survey of the available biochemical and electron microscopic data on keyhole limpet hemocyanin (KLH). RESULTS: The biosynthesis of KLH and its biological role are discussed and the purification of the two isoforms of KLH (KLH1 and KLH2) presented in some detail. The determination of the molecular mass of KLH, its functional unit structure, carbohydrate content, immunological analysis and aspects of the molecular biology of KLH are all dealt with. Transmission electron microscopy (TEM) and crossed immunoelectrophoresis have played a significant part in the understanding of KLH structure. We present a summary of TEM studies on the native oligomers of KLH, the experimental manipulation of the different oligomeric states, immunological analysis and subunit reassociation. CONCLUSION: This fundamental structural information provides the scientific background upon which the understanding of the in vivo immunostimulatory function of KLH can be based. Copyright 2000 S. Karger AG, Basel
OBJECTIVES: In this short review we present a survey of the available biochemical and electron microscopic data on keyhole limpet hemocyanin (KLH). RESULTS: The biosynthesis of KLH and its biological role are discussed and the purification of the two isoforms of KLH (KLH1 and KLH2) presented in some detail. The determination of the molecular mass of KLH, its functional unit structure, carbohydrate content, immunological analysis and aspects of the molecular biology of KLH are all dealt with. Transmission electron microscopy (TEM) and crossed immunoelectrophoresis have played a significant part in the understanding of KLH structure. We present a summary of TEM studies on the native oligomers of KLH, the experimental manipulation of the different oligomeric states, immunological analysis and subunit reassociation. CONCLUSION: This fundamental structural information provides the scientific background upon which the understanding of the in vivo immunostimulatory function of KLH can be based. Copyright 2000 S. Karger AG, Basel
Authors: Susana Inoges; Ascension Lopez-Diaz de Cerio; Helena Villanueva; Fernando Pastor; Elena Soria; Maurizio Bendandi Journal: World J Clin Oncol Date: 2011-06-10
Authors: Lyudmila Velkova; Pavlina Dolashka; Bernhard Lieb; Aleksander Dolashki; Wolfgang Voelter; Jozef Van Beeumen; Bart Devreese Journal: Glycoconj J Date: 2011-06-10 Impact factor: 2.916
Authors: Bernhard Lieb; Wolfgang Gebauer; Christos Gatsogiannis; Frank Depoix; Nadja Hellmann; Myroslaw G Harasewych; Ellen E Strong; Jürgen Markl Journal: Front Zool Date: 2010-05-13 Impact factor: 3.172