Literature DB >> 10821633

Epitope mapping of LB509, a monoclonal antibody directed against human alpha-synuclein.

R Jakes1, R A Crowther, V M Lee, J Q Trojanowski, T Iwatsubo, M Goedert.   

Abstract

Alpha-synuclein is a 140 amino acid protein that forms the major component of the abnormal filaments that make up the Lewy bodies and Lewy neurites of Parkinson's disease and dementia with Lewy bodies. It is also the major component of the filamentous glial cytoplasmic inclusions of multiple system atrophy. Here we have used recombinant alpha-synucleins and peptide competition to show that the monoclonal anti-alpha-synuclein antibody LB509 recognizes amino acids 115-122 of human alpha-synuclein. The antibody strongly labelled filaments extracted from multiple system atrophy brain, showing the presence of residues 115-122 of alpha-synuclein. LB509 failed to react with mouse, rat and zebra finch alpha-synuclein, because of amino acid differences with human alpha-synuclein. Since LB509 recognizes human but not rodent alpha-synuclein, it will be a useful reagent for the characterization of mouse lines transgenic for human alpha-synuclein.

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Year:  1999        PMID： 10821633     DOI： 10.1016/s0304-3940(99)00411-5

Source DB:  PubMed          Journal:  Neurosci Lett        ISSN： 0304-3940            Impact factor:   3.046


  33 in total

1.  Fiber diffraction of synthetic alpha-synuclein filaments shows amyloid-like cross-beta conformation.

Authors:  L C Serpell; J Berriman; R Jakes; M Goedert; R A Crowther
Journal:  Proc Natl Acad Sci U S A       Date:  2000-04-25       Impact factor: 11.205

2.  Neuropathology in mice expressing human alpha-synuclein.

Authors:  H van der Putten; K H Wiederhold; A Probst; S Barbieri; C Mistl; S Danner; S Kauffmann; K Hofele; W P Spooren; M A Ruegg; S Lin; P Caroni; B Sommer; M Tolnay; G Bilbe
Journal:  J Neurosci       Date:  2000-08-15       Impact factor: 6.167

3.  Neurodegeneration with brain iron accumulation, type 1 is characterized by alpha-, beta-, and gamma-synuclein neuropathology.

Authors:  J E Galvin; B Giasson; H I Hurtig; V M Lee; J Q Trojanowski
Journal:  Am J Pathol       Date:  2000-08       Impact factor: 4.307

4.  Synphilin-1-binding protein NUB1 is colocalized with nonfibrillar, proteinase K-resistant α-synuclein in presynapses in Lewy body disease.

Authors:  Kunikazu Tanji; Fumiaki Mori; Katsumi Kito; Akiyoshi Kakita; Junsei Mimura; Ken Itoh; Hitoshi Takahashi; Tetsu Kamitani; Koichi Wakabayashi
Journal:  J Neuropathol Exp Neurol       Date:  2011-10       Impact factor: 3.685

5.  Alterations in axonal transport motor proteins in sporadic and experimental Parkinson's disease.

Authors:  Yaping Chu; Gerardo A Morfini; Lori B Langhamer; Yinzhen He; Scott T Brady; Jeffrey H Kordower
Journal:  Brain       Date:  2012-06-19       Impact factor: 13.501

6.  Aggregation of α-synuclein in S. cerevisiae is associated with defects in endosomal trafficking and phospholipid biosynthesis.

Authors:  James H Soper; Victoria Kehm; Christopher G Burd; Vytas A Bankaitis; Virginia M-Y Lee
Journal:  J Mol Neurosci       Date:  2010-10-02       Impact factor: 3.444

7.  Quantitative proteomics identifies surfactant-resistant alpha-synuclein in cerebral cortex of Parkinsonism-dementia complex of Guam but not Alzheimer's disease or progressive supranuclear palsy.

Authors:  Wan Yang; Randall L Woltjer; Izabela Sokal; Catherine Pan; Yan Wang; Mary Brodey; Elaine R Peskind; James B Leverenz; Jing Zhang; Daniel P Perl; Douglas R Galasko; Thomas J Montine
Journal:  Am J Pathol       Date:  2007-08-03       Impact factor: 4.307

8.  Nurr1 in Parkinson's disease and related disorders.

Authors:  Yaping Chu; Weidong Le; Katie Kompoliti; Joseph Jankovic; Elliott J Mufson; Jeffrey H Kordower
Journal:  J Comp Neurol       Date:  2006-01-20       Impact factor: 3.215

9.  Alpha-synuclein aggregation induced by brief ischemia negatively impacts neuronal survival in vivo: a study in [A30P]alpha-synuclein transgenic mouse.

Authors:  Isin Unal-Cevik; Yasemin Gursoy-Ozdemir; Muge Yemisci; Sevda Lule; Gunfer Gurer; Alp Can; Veronica Müller; Philip J Kahle; Turgay Dalkara
Journal:  J Cereb Blood Flow Metab       Date:  2010-09-29       Impact factor: 6.200

10.  Lewy body pathology in familial Alzheimer disease: evidence for disease- and mutation-specific pathologic phenotype.

Authors:  James B Leverenz; Mark A Fishel; Elaine R Peskind; Thomas J Montine; David Nochlin; Ellen Steinbart; Murray A Raskind; Gerard D Schellenberg; Thomas D Bird; Debby Tsuang
Journal:  Arch Neurol       Date:  2006-03
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