OBJECTIVES: The use of high-resolution in vivo confocal imaging may offer a clinically useful adjunct to standard methods for the diagnosis and screening of epithelial precancers. This study assesses the feasibility of real-time confocal reflectance imaging of cervical tissue and the use of acetic acid as a contrast agent to increase visualization of cell nuclei. STUDY DESIGN: A confocal microscope was used to image cervical cells and colposcopically normal and abnormal cervical biopsy specimens. Images were obtained before and after the application of 6% acetic acid. RESULTS: The confocal imaging system resolved subcellular detail throughout the entire epithelial thickness. Normal and abnormal tissues were clearly able to be differentiated. Addition of acetic acid enhanced nuclear signal in all acquired images. CONCLUSION: High-contrast reflected light images of cervical tissue are attainable in near real time. Acetic acid significantly increases light scattering from cell nuclei, which may partially explain why acetowhitening occurs.
OBJECTIVES: The use of high-resolution in vivo confocal imaging may offer a clinically useful adjunct to standard methods for the diagnosis and screening of epithelial precancers. This study assesses the feasibility of real-time confocal reflectance imaging of cervical tissue and the use of acetic acid as a contrast agent to increase visualization of cell nuclei. STUDY DESIGN: A confocal microscope was used to image cervical cells and colposcopically normal and abnormal cervical biopsy specimens. Images were obtained before and after the application of 6% acetic acid. RESULTS: The confocal imaging system resolved subcellular detail throughout the entire epithelial thickness. Normal and abnormal tissues were clearly able to be differentiated. Addition of acetic acid enhanced nuclear signal in all acquired images. CONCLUSION: High-contrast reflected light images of cervical tissue are attainable in near real time. Acetic acid significantly increases light scattering from cell nuclei, which may partially explain why acetowhitening occurs.
Authors: Osman O Ahsen; Yuankai K Tao; Benjamin M Potsaid; Yuri Sheikine; James Jiang; Ireneusz Grulkowski; Tsung-Han Tsai; Vijaysekhar Jayaraman; Martin F Kraus; James L Connolly; Joachim Hornegger; Alex Cable; James G Fujimoto Journal: Opt Express Date: 2013-07-29 Impact factor: 3.894
Authors: Alicia L Carlson; Ann M Gillenwater; Michelle D Williams; Adel K El-Naggar; R R Richards-Kortum Journal: Technol Cancer Res Treat Date: 2007-10
Authors: Nima Tabatabaei; Dongkyun Kang; Tao Wu; Minkyu Kim; Robert W Carruth; John Leung; Jenny S Sauk; Wayne Shreffler; Qian Yuan; Aubrey Katz; Norman S Nishioka; Guillermo J Tearney Journal: Biomed Opt Express Date: 2013-12-13 Impact factor: 3.732