Literature DB >> 10818365

Oxidation of selenomethionine: some MADness in the method!

A J Sharff1, E Koronakis, B Luisi, V Koronakis.   

Abstract

Since it was first reported, the multiwavelength anomalous diffraction (MAD) technique for the determination of protein structures has become widely accepted and increasingly popular. Here, it is demonstrated that the anomalous signal from selenomethione (SeMet) substituted proteins can be significantly enhanced by oxidation.

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Year:  2000        PMID: 10818365     DOI: 10.1107/s090744490000370x

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN: 0907-4449


  7 in total

1.  Human fatty acid synthase: structure and substrate selectivity of the thioesterase domain.

Authors:  Bornali Chakravarty; Ziwei Gu; Subrahmanyam S Chirala; Salih J Wakil; Florante A Quiocho
Journal:  Proc Natl Acad Sci U S A       Date:  2004-10-26       Impact factor: 11.205

Review 2.  Selenium incorporation using recombinant techniques.

Authors:  Helen Walden
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-03-24

3.  Crystal structure of saposin B reveals a dimeric shell for lipid binding.

Authors:  Victoria E Ahn; Kym F Faull; Julian P Whitelegge; Arvan L Fluharty; Gilbert G Privé
Journal:  Proc Natl Acad Sci U S A       Date:  2002-12-23       Impact factor: 11.205

4.  Insights into MHC class I peptide loading from the structure of the tapasin-ERp57 thiol oxidoreductase heterodimer.

Authors:  Gang Dong; Pamela A Wearsch; David R Peaper; Peter Cresswell; Karin M Reinisch
Journal:  Immunity       Date:  2009-01-16       Impact factor: 31.745

5.  Pseudosymmetry, high copy number and twinning complicate the structure determination of Desulfovibrio desulfuricans (ATCC 29577) flavodoxin.

Authors:  Megan Guelker; Loren Stagg; Pernilla Wittung-Stafshede; Yousif Shamoo
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2009-05-15

6.  Structural changes of envelope proteins during alphavirus fusion.

Authors:  Long Li; Joyce Jose; Ye Xiang; Richard J Kuhn; Michael G Rossmann
Journal:  Nature       Date:  2010-12-02       Impact factor: 49.962

7.  Structure of the outer membrane complex of a type IV secretion system.

Authors:  Vidya Chandran; Rémi Fronzes; Stéphane Duquerroy; Nora Cronin; Jorge Navaza; Gabriel Waksman
Journal:  Nature       Date:  2009-11-29       Impact factor: 49.962

  7 in total

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