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Literature DB >> 1081384

Cytoplasmic and outer membranes separation in Rhodopseudomonas sphaeroides.

Abstract

A cell envelope fraction had been prepared after mechanical disruption of lysozyme-EDTA spheroplasts from depigmented Rhodopseudomonas sphaeroides (aerobically grown in the light). On linear sucrose gradients this fraction can be separated in a cytoplasmic membrane fraction and an outer membrane fraction. The cytoplasmic fraction (buoyant density: 1.18 g/cm3) has been characterized by its succinic dehydrogenase activity and by its composition. The outer membrane fraction (buoyant density: 1.21 g/cm3) does not contain any respiratory activity nor hemoproteins. The same fractionation has been done on cells repigmented in the dark by lowering the O2 pressure. In that case the same two fractions have been detected in addition to the chromatophore fraction (buoyant density: 1.14 g/cm3). However both, and specially the outer membrane fraction, were contaminated by chromatophore material.

Entities: Chemical Species

Mesh：

Substances：

Year: 1975 PMID： 1081384 DOI： 10.1007/bf00447146

Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552

32 in total

1. Carotenoid pigment conversion in Rhodopseudomonas spheroides.

Authors: E A SHNEOUR
Journal: Biochim Biophys Acta Date: 1962-08-27

2. A method for determining the concentration of ubiquinone in mitochondrial preparations.

Authors: A M PUMPHREY; E R REDFEARN
Journal: Biochem J Date: 1960-07 Impact factor: 3.857

3. Outer membrane proteins of Escherichia coli. II. Heterogeneity of major outer membrane polypeptides.

Authors: C A Schnaitman
Journal: Arch Biochem Biophys Date: 1973-08 Impact factor: 4.013

4. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

Authors: M J Osborn; J E Gander; E Parisi; J Carson
Journal: J Biol Chem Date: 1972-06-25 Impact factor: 5.157

8. Photosynthetic membrane development in Rhodopseudomonas spheroides: incorporation of bacteriochlorophyll and development of energy transfer and photochemical activity.

Authors: R A Cellarius; G A Peters
Journal: Biochim Biophys Acta Date: 1969-10-21

3. Assessing the precision of high-throughput computational and laboratory approaches for the genome-wide identification of protein subcellular localization in bacteria.

Authors: Sébastien Rey; Jennifer L Gardy; Fiona S L Brinkman
Journal: BMC Genomics Date: 2005-11-17 Impact factor: 3.969

3 in total

Cytoplasmic and outer membranes separation in Rhodopseudomonas sphaeroides.

1. Carotenoid pigment conversion in Rhodopseudomonas spheroides.

2. A method for determining the concentration of ubiquinone in mitochondrial preparations.

3. Outer membrane proteins of Escherichia coli. II. Heterogeneity of major outer membrane polypeptides.

4. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.

5. Isolation and fractionation of the photosynthetic membranous organelles from Rhodopseudomonas spheroides.

6. Iron-containing proteins in Chromatium. I. Solubilization of membrane-bound cytochrome.

7. The separation of chromatophores from the cell envelope in Rhodopseudomonas spheroides.

8. Photosynthetic membrane development in Rhodopseudomonas spheroides: incorporation of bacteriochlorophyll and development of energy transfer and photochemical activity.

9. Membranes of Rhodopseudomonas spheroides: interactions of chromatophores with the cell envelope.

10. THE PREPARATION AND PROPERTIES OF BACTERIAL CHROMATOPHORE FRACTIONS.

1. Penicillin-binding proteins of Rhodopseudomonas sphaeroides and their membrane localization.

2. Protein composition of Rhodopseudomonas sphaeroides outer membrane.

3. Assessing the precision of high-throughput computational and laboratory approaches for the genome-wide identification of protein subcellular localization in bacteria.