OBJECTIVE: IL-8 is thought to be the principal chemokine responsible for neutrophil activation and tissue infiltration in patients with Behçet's disease (BD). In various studies serum levels of IL-8 were reported to be increased. METHODS: IL-8 mRNA was purified from both peripheral whole blood samples and separated lymphocytes, granulocytes and monocytes of patients with BD and compared to that from healthy (HC) and disease controls. IL-8 sequences were revealed by PCR amplification using appropriate sequence-specific primers. mRNA levels were determined semi-quantitatively using an image analyser in comparison with beta-actin. RESULTS: Although the differences did not reach statistical significance, BD patients tended to have higher IL-8 mRNA levels compared to HC in whole blood samples (2.0 +/- 1.4 vs 1.5 +/- 1.2) as well as in their lymphocytes (2.7 +/- 1.6 vs 1.5 +/- 0.9). No differences were observed between BD and HC in monocyte or granulocyte IL-8 mRNA levels. CONCLUSION: Our results suggest that the cellular source of IL-8 is diverse in BD with a possible major contribution by lymphocytes.
OBJECTIVE:IL-8 is thought to be the principal chemokine responsible for neutrophil activation and tissue infiltration in patients with Behçet's disease (BD). In various studies serum levels of IL-8 were reported to be increased. METHODS:IL-8 mRNA was purified from both peripheral whole blood samples and separated lymphocytes, granulocytes and monocytes of patients with BD and compared to that from healthy (HC) and disease controls. IL-8 sequences were revealed by PCR amplification using appropriate sequence-specific primers. mRNA levels were determined semi-quantitatively using an image analyser in comparison with beta-actin. RESULTS: Although the differences did not reach statistical significance, BD patients tended to have higher IL-8 mRNA levels compared to HC in whole blood samples (2.0 +/- 1.4 vs 1.5 +/- 1.2) as well as in their lymphocytes (2.7 +/- 1.6 vs 1.5 +/- 0.9). No differences were observed between BD and HC in monocyte or granulocyte IL-8 mRNA levels. CONCLUSION: Our results suggest that the cellular source of IL-8 is diverse in BD with a possible major contribution by lymphocytes.
Authors: Simone Schmid; Petra C Kuechler; Markus Britschgi; Urs C Steiner; Nikhil Yawalkar; Alain Limat; Kurt Baltensperger; Lasse Braathen; Werner J Pichler Journal: Am J Pathol Date: 2002-12 Impact factor: 4.307
Authors: Y Imamura; M S Kurokawa; H Yoshikawa; K Nara; E Takada; C Masuda; S Tsukikawa; S Ozaki; T Matsuda; N Suzuki Journal: Clin Exp Immunol Date: 2005-02 Impact factor: 4.330
Authors: Giuseppe Lopalco; Orso Maria Lucherini; Antonio Vitale; Rosaria Talarico; Antonio Lopalco; Mauro Galeazzi; Giovanni Lapadula; Luca Cantarini; Florenzo Iannone Journal: Medicine (Baltimore) Date: 2015-10 Impact factor: 1.817