Literature DB >> 10806588

Sex chromosomal analysis of spermatozoa from infertile men using fluorescence in situ hybridization.

N Nishikawa1, I Murakami, K Ikuta, K Suzumori.   

Abstract

PURPOSE: To confirm an association between male infertility and chromosome aberrations of spermatozoa, we demonstrated the frequency of numerical abnormalities of spermatozoa from infertile men with abnormal semen parameters compared with fertile controls.
METHOD: Sperm cells from 10 infertile patients were investigated for disomy rates of sex chromosomes and chromosome 18 and diploidy by fluorescence in situ hybridization (FISH). All patients showed oligoasthenozoospermia with sperm counts 3-20 x 10(6)/ml and motile rates 0-40%.
RESULTS: Regarding XY disomy, a significantly higher frequency was found in 8 of 10 patients as compared to normal fertile men. The disomy rates of chromosome 18, XX, YY, and diploidy rate were not increased.
CONCLUSIONS: There is an association between male infertility and embryo with aneuploidy of sex chromosomes. Counseling about possible genetic risks should be provided to the infertile couples planning assisted reproduction treatment.

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Mesh:

Year:  2000        PMID: 10806588      PMCID: PMC3455159          DOI: 10.1023/a:1009413916753

Source DB:  PubMed          Journal:  J Assist Reprod Genet        ISSN: 1058-0468            Impact factor:   3.412


  31 in total

1.  Sephadex filtration and human serum albumin gradients do not select spermatozoa by sex chromosome: a fluorescent in-situ hybridization study.

Authors:  F Vidal; M Moragas; V Català; M J Torelló; J Santaló; G Calderón; C Gimenez; P N Barri; J Egozcue; A Veiga
Journal:  Hum Reprod       Date:  1993-10       Impact factor: 6.918

2.  Simultaneous detection of X- and Y-bearing human sperm by double fluorescence in situ hybridization.

Authors:  T L Han; J H Ford; G C Webb; S P Flaherty; A Correll; C D Matthews
Journal:  Mol Reprod Dev       Date:  1993-03       Impact factor: 2.609

3.  Non-disjunction in human sperm: results of fluorescence in situ hybridization studies using two and three probes.

Authors:  B J Williams; C A Ballenger; H E Malter; F Bishop; M Tucker; T A Zwingman; T J Hassold
Journal:  Hum Mol Genet       Date:  1993-11       Impact factor: 6.150

4.  Incidence of chromosome 18 disomy in human sperm nuclei as detected by nonisotopic in situ hybridization.

Authors:  M Guttenbach; R Schakowski; M Schmid
Journal:  Hum Genet       Date:  1994-04       Impact factor: 4.132

5.  Meiotic studies in a series of 1100 infertile and sterile males.

Authors:  J Egozcue; C Templado; F Vidal; J Navarro; F Morer-Fargas; S Marina
Journal:  Hum Genet       Date:  1983       Impact factor: 4.132

6.  Direct analysis of the chromosome constitution of human spermatozoa.

Authors:  E Rudak; P A Jacobs; R Yanagimachi
Journal:  Nature       Date:  1978-08-31       Impact factor: 49.962

7.  Detection of aneuploidy for chromosomes 4, 6, 7, 8, 9, 10, 11, 12, 13, 17, 18, 21, X and Y by fluorescence in-situ hybridization in spermatozoa from nine patients with oligoasthenoteratozoospermia undergoing intracytoplasmic sperm injection.

Authors:  M G Pang; S F Hoegerman; A J Cuticchia; S Y Moon; G F Doncel; A A Acosta; W G Kearns
Journal:  Hum Reprod       Date:  1999-05       Impact factor: 6.918

8.  Achievement of meiosis in XXY germ cells: study of 543 sperm karyotypes from an XY/XXY mosaic patient.

Authors:  J Cozzi; E Chevret; S Rousseaux; R Pelletier; V Benitz; H Jalbert; B Sèle
Journal:  Hum Genet       Date:  1994-01       Impact factor: 4.132

9.  Detection of aneuploid human sperm by fluorescence in situ hybridization: evidence for a donor difference in frequency of sperm disomic for chromosomes 1 and Y.

Authors:  W A Robbins; R Segraves; D Pinkel; A J Wyrobek
Journal:  Am J Hum Genet       Date:  1993-04       Impact factor: 11.025

10.  Aneuploidy detection in human sperm nuclei using fluorescence in situ hybridization.

Authors:  J M Holmes; R H Martin
Journal:  Hum Genet       Date:  1993-03       Impact factor: 4.132

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  4 in total

1.  The association between sperm sex chromosome disomy and semen concentration, motility and morphology.

Authors:  M E McAuliffe; P L Williams; S A Korrick; R Dadd; M J Perry
Journal:  Hum Reprod       Date:  2012-08-14       Impact factor: 6.918

2.  Detection of aneuploidy rate for chromosomes X, Y and 8 by fluorescence in-situ hybridization in spermatozoa from patients with severe non-obstructive oligozoospermia.

Authors:  Soumaya Mougou-Zerelli; Sonia Brahem; Molka Kammoun; Mehdi Jerbi; Hatem Elghezal; Mounir Ajina; Ali Saad
Journal:  J Assist Reprod Genet       Date:  2011-08-19       Impact factor: 3.412

3.  The association between male infertility and sperm disomy: evidence for variation in disomy levels among individuals and a correlation between particular semen parameters and disomy of specific chromosome pairs.

Authors:  Helen G Tempest; Sheryl T Homa; Maria Dalakiouridou; Dimitra Christopikou; David Wright; Xiao P Zhai; Darren K Griffin
Journal:  Reprod Biol Endocrinol       Date:  2004-12-14       Impact factor: 5.211

Review 4.  Cytogenetic determinants of male fertility.

Authors:  R H Martin
Journal:  Hum Reprod Update       Date:  2008-06-04       Impact factor: 15.610

  4 in total

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