| Literature DB >> 10806305 |
Y Noguchi1, A Saito, Y Miyagi, S Yamanaka, D Marat, C Doi, T Yoshikawa, A Tsuburaya, T Ito, S Satoh.
Abstract
We attempted to suppress glucose transporter 1 (GLUT1) expression by transfecting MKN45 cells with cDNA for antisense GLUT1. Glucose transport was significantly decreased in cells with antisense GLUT1 compared with wild-type cells or cells with vector alone. Suppression of GLUT1 mRNA resulted in a decreased number of cells in the S phase. This was accompanied by overexpression of p21 protein. Tumorigenicity in the nude mice injected with antisense GLUT1 expressing cells was significantly slower than in those with wild-type MKN45 cells. These results suggest that antisense GLUT1 mRNA inhibits tumor growth through a G(1) arrest and that expression of antisense GLUT1 mRNA via gene therapy can be used as a tool in the treatment of cancer.Entities:
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Year: 2000 PMID： 10806305 DOI： 10.1016/s0304-3835(00)00392-x
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679