Analysis of glycoprotein-derived glycopeptides.

Glycosylation of proteins represents one of the most important post-(co-)translational events in view of the ubiquity of the phenomenon. In most cases, the covalently linked glycans are involved in the functioning of these biomolecules in biological systems. Detailed information on the carbohydrate moieties including monosaccharide composition, anomeric configurations, type of glycosidic linkages and attachment sites at the protein is indispensable in describing the ultimate structure of a specific glycoprotein. This chapter presents a general strategy for the structural characterization of glycoproteins/glycopeptides focussed on the glycan part. Some of the techniques commonly used, like enzyme treatments, separation methods, chemical analyses, mass spectrometry and nuclear magnetic resonance spectroscopy are briefly reviewed.