Literature DB >> 10799984

Power consumption in shaking flasks on rotary shaking machines: II. Nondimensional description of specific power consumption and flow regimes in unbaffled flasks at elevated liquid viscosity.

J Büchs1, U Maier, C Milbradt, B Zoels.   

Abstract

This article is the second part of a series presenting and modeling the hydrodynamics and specific power consumption in shaking flasks on rotary (orbital) shaking machines. In part I, a new method was introduced that enables the accurate determination of the specific power consumption in shaking flasks. The method was first applied to investigate unbaffled flasks with a nominal volume of < or =1 L at low viscosity. In part II, the results for the specific power consumption of unbaffled shaking flasks at elevated viscosities are investigated after varying shaking frequency, flask size, filling volume, and shaking diameter. The theory introduced in part I is extended to liquids of elevated viscosities using nondimensional equations. With these results, the specific power consumption in unbaffled shaking flasks can now be fully described. For the first time, the phenomenon of the liquid being "out of phase" is observed and described. This occurs at certain operating conditions and is characterized by an increasing amount of liquid not following the movement of the shaking table, thus reducing the specific power consumption. This, of course, has much relevance for practical work with microbial cultures. The phenomenon of being "out-of-phase" is described in the form of a newly defined nondimensional phase number (Ph) in analogy to a partially filled, rotating horizontal drum. The Ph can be used to determine reasonable operating conditions for shaking flask experiments when using viscous media, avoiding unfavorable "out-of-phase" operation. Copyright 2000 John Wiley & Sons, Inc.

Mesh:

Year:  2000        PMID: 10799984

Source DB:  PubMed          Journal:  Biotechnol Bioeng        ISSN: 0006-3592            Impact factor:   4.530


  20 in total

1.  Methods for intense aeration, growth, storage, and replication of bacterial strains in microtiter plates.

Authors:  W A Duetz; L Rüedi; R Hermann; K O'Connor; J Büchs; B Witholt
Journal:  Appl Environ Microbiol       Date:  2000-06       Impact factor: 4.792

2.  The oxygen transfer rate as key parameter for the characterization of Hansenula polymorpha screening cultures.

Authors:  Christoph Stöckmann; Ulrike Maier; Tibor Anderlei; Christof Knocke; Gerd Gellissen; Jochen Büchs
Journal:  J Ind Microbiol Biotechnol       Date:  2003-10-28       Impact factor: 3.346

3.  An experimental comparison of respiration measuring techniques in fermenters and shake flasks: exhaust gas analyzer vs. RAMOS device vs. respirometer.

Authors:  Juri M Seletzky; Ute Noack; Sebastian Hahn; Arnd Knoll; Ghassem Amoabediny; Jochen Büchs
Journal:  J Ind Microbiol Biotechnol       Date:  2006-09-26       Impact factor: 3.346

4.  A high gas fraction, reduced power, syngas bioprocessing method demonstrated with a Clostridium ljungdahlii OTA1 paper biocomposite.

Authors:  Mark J Schulte; Jeff Wiltgen; John Ritter; Charles B Mooney; Michael C Flickinger
Journal:  Biotechnol Bioeng       Date:  2016-03-28       Impact factor: 4.530

5.  Contribution of the Klebsiella pneumoniae capsule to bacterial aggregate and biofilm microstructures.

Authors:  Stephen P Dzul; Margaret M Thornton; Danial N Hohne; Elizabeth J Stewart; Aayush A Shah; David M Bortz; Michael J Solomon; John G Younger
Journal:  Appl Environ Microbiol       Date:  2011-01-14       Impact factor: 4.792

6.  A comprehensive comparison of mixing and mass transfer in shake flasks and their relationship with MAb productivity of CHO cells.

Authors:  Saumel Pérez-Rodriguez; Greta I Reynoso-Cereceda; Norma A Valdez-Cruz; Mauricio A Trujillo-Roldán
Journal:  Bioprocess Biosyst Eng       Date:  2022-03-26       Impact factor: 3.210

7.  Scale-up from shake flasks to bioreactor, based on power input and Streptomyces lividans morphology, for the production of recombinant APA (45/47 kDa protein) from Mycobacterium tuberculosis.

Authors:  Ramsés A Gamboa-Suasnavart; Luz D Marín-Palacio; José A Martínez-Sotelo; Clara Espitia; Luis Servín-González; Norma A Valdez-Cruz; Mauricio A Trujillo-Roldán
Journal:  World J Microbiol Biotechnol       Date:  2013-03-10       Impact factor: 3.312

8.  The O-mannosylation and production of recombinant APA (45/47 KDa) protein from Mycobacterium tuberculosis in Streptomyces lividans is affected by culture conditions in shake flasks.

Authors:  Ramsés A Gamboa-Suasnavart; Norma A Valdez-Cruz; Laura E Cordova-Dávalos; José A Martínez-Sotelo; Luis Servín-González; Clara Espitia; Mauricio A Trujillo-Roldán
Journal:  Microb Cell Fact       Date:  2011-12-20       Impact factor: 5.328

9.  Characterization of hydrodynamics and volumetric power input in microtiter plates for the scale-up of downstream operations.

Authors:  Ignacio Montes-Serrano; Peter Satzer; Alois Jungbauer; Astrid Dürauer
Journal:  Biotechnol Bioeng       Date:  2021-11-12       Impact factor: 4.395

Review 10.  Oxygen transfer characteristics of miniaturized bioreactor systems.

Authors:  Timothy V Kirk; Nicolas Szita
Journal:  Biotechnol Bioeng       Date:  2013-01-17       Impact factor: 4.530

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