Literature DB >> 10799470

Clonal analysis of the human B-cell repertoire using a heteromobility assay.

D Tewari1.   

Abstract

A heteromobility duplex tracking assay was developed to analyze B-cell clonality. The assay was based on the genetic variability of B-cell immunoglobulin (Ig) sequences. Binding of amplified (Ig) sequences to a single-stranded radiolabeled Ig DNA probe resulted in the formation of heteroduplexes. The mobilities of these heteroduplexes helped to distinguish clonal B cells.

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Year:  2000        PMID： 10799470      PMCID： PMC95903          DOI： 10.1128/CDLI.7.3.507-509.2000

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  12 in total

1.  An improved PCR-heteroduplex method permits high-sensitivity detection of clonal expansions in complex T cell populations.

Authors:  A Wack; D Montagna; P Dellabona; G Casorati
Journal:  J Immunol Methods       Date:  1996-09-27       Impact factor: 2.303

2.  High-resolution analysis of T-cell receptor beta-chain repertoires using DNA heteroduplex tracking: generally stable, clonal CD8+ expansions in all healthy young adults.

Authors:  D F Shen; L Doukhan; S Kalams; E Delwart
Journal:  J Immunol Methods       Date:  1998-06-01       Impact factor: 2.303

3.  Analysis of the heavy chain repertoire of human peripheral B cells using single-cell polymerase chain reaction.

Authors:  H P Brezinschek; R I Brezinschek; P E Lipsky
Journal:  J Immunol       Date:  1995-07-01       Impact factor: 5.422

4.  Immunological and virological analyses of persons infected by human immunodeficiency virus type 1 while participating in trials of recombinant gp120 subunit vaccines.

Authors:  R I Connor; B T Korber; B S Graham; B H Hahn; D D Ho; B D Walker; A U Neumann; S H Vermund; J Mestecky; S Jackson; E Fenamore; Y Cao; F Gao; S Kalams; K J Kunstman; D McDonald; N McWilliams; A Trkola; J P Moore; S M Wolinsky
Journal:  J Virol       Date:  1998-02       Impact factor: 5.103

5.  Heteroduplex analysis of VDJ amplified segments from rearranged IgH genes for clonality assessments in B-cell non-Hodgkin's lymphoma. A comparison between different strategies.

Authors:  M González; D González; R López-Pérez; R García-Sanz; M C Chillón; A Balanzategui; M V Mateos; I Alaejos; A W Langerak; A Orfão; J J Van Dongen; J F San Miguel
Journal:  Haematologica       Date:  1999-09       Impact factor: 9.941

Review 6.  Clonal dominance: cause for a limited and failing immune response to HIV-1 infection and vaccination.

Authors:  H Kohler; J Goudsmit; P Nara
Journal:  J Acquir Immune Defic Syndr (1988)       Date:  1992

7.  Diversity of the T-cell receptor BV repertoire in HIV-1-infected patients reflects the biphasic CD4+ T-cell repopulation kinetics during highly active antiretroviral therapy.

Authors:  S Kostense; F M Raaphorst; D W Notermans; J Joling; B Hooibrink; N G Pakker; S A Danner; J M Teale; F Miedema
Journal:  AIDS       Date:  1998-12-24       Impact factor: 4.177

8.  Evidence of antigen receptor-influenced oligoclonal B lymphocyte expansion in the synovium of a patient with longstanding rheumatoid arthritis.

Authors:  S K Lee; S L Bridges; P M Kirkham; W J Koopman; H W Schroeder
Journal:  J Clin Invest       Date:  1994-01       Impact factor: 14.808

9.  PCR-based analysis of the murine immunoglobulin heavy-chain repertoire.

Authors:  S Delassus; A Gey; S Darche; A Cumano; C Roth; P Kourilsky
Journal:  J Immunol Methods       Date:  1995-08-18       Impact factor: 2.303

10.  Profile of immunoglobulin heavy chain variable gene repertoires and highly selective detection of malignant clonotypes in acute lymphoblastic leukemia.

Authors:  S J Greenberg; Y Choi; M Ballow; T L Du; P M Ward; M H Rickert; S Frankel; S H Bernstein; M L Brecher
Journal:  J Leukoc Biol       Date:  1995-06       Impact factor: 4.962
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  1 in total

Review 1.  From immunoglobulin gene fingerprinting to motif-specific hybridization: advances in the analysis of B lymphoid clonality in rheumatic diseases.

Authors:  Jan Voswinkel; Angela Gause
Journal:  Arthritis Res       Date:  2001-09-26
  1 in total

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