Literature DB >> 10795720

Glyceraldehyde-3-phosphate dehydrogenase varies with age in glycolytic muscles of rats.

D A Lowe1, H Degens, K D Chen, S E Alway.   

Abstract

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA, protein, and enzyme activity levels in hindlimb muscles of adult and senescent Fischer 344 x Brown Norway rats were investigated. Soleus muscles from adult and senescent rats had similar levels of GAPDH. In contrast, muscles containing a large proportion of glycolytic fibers had lower GAPDH levels in senescent rats relative to these muscles in adult rats; this was observed at both the mRNA and protein levels. These data indicate that skeletal muscle glycolytic capacity of fast muscles is diminished with age and that it may be caused by changes at the level of transcription. Also, because GAPDH mRNA levels change with age in several rat muscles, GAPDH mRNA is not always a proper internal control for mRNA analyses of aging skeletal muscle.

Entities:  

Mesh：

Substances：

Year:  2000        PMID： 10795720     DOI： 10.1093/gerona/55.3.b160

Source DB:  PubMed          Journal:  J Gerontol A Biol Sci Med Sci        ISSN： 1079-5006            Impact factor:   6.053


  19 in total

1.  Molecular and quantitative characterisation of the porcine embryonic myosin heavy chain gene.

Authors:  Y M Sun; N da Costa; R Birrell; A L Archibald; H Alzuherri; K C Chang
Journal:  J Muscle Res Cell Motil       Date:  2001       Impact factor: 2.698

2.  Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Advantages of Stain-Free Technology.

Authors:  Rômulo Leão Silva Neris; Andrea Marie Chua Dobles; Aldrin V Gomes
Journal:  Methods Mol Biol       Date:  2021

3.  The impact of age and frailty on ventricular structure and function in C57BL/6J mice.

Authors:  H A Feridooni; A E Kane; O Ayaz; A Boroumandi; N Polidovitch; R G Tsushima; R A Rose; S E Howlett
Journal:  J Physiol       Date:  2017-05-14       Impact factor: 5.182

4.  V3 stain-free workflow for a practical, convenient, and reliable total protein loading control in western blotting.

Authors:  Anton Posch; Jonathan Kohn; Kenneth Oh; Matt Hammond; Ning Liu
Journal:  J Vis Exp       Date:  2013-12-30       Impact factor: 1.355

5.  Ca2+ dependency of limb muscle fiber contractile mechanics in young and older adults.

Authors:  Laura E Teigen; Christopher W Sundberg; Lauren J Kelly; Sandra K Hunter; Robert H Fitts
Journal:  Am J Physiol Cell Physiol       Date:  2020-04-29       Impact factor: 4.249

6.  Increased myogenic repressor Id mRNA and protein levels in hindlimb muscles of aged rats.

Authors:  Stephen E Alway; Hans Degens; Dawn A Lowe; Gururaj Krishnamurthy
Journal:  Am J Physiol Regul Integr Comp Physiol       Date:  2002-02       Impact factor: 3.619

7.  Signal transduction in Alzheimer disease: p21-activated kinase signaling requires C-terminal cleavage of APP at Asp664.

Authors:  Thuy-Vi V Nguyen; Veronica Galvan; Wei Huang; Surita Banwait; Huidong Tang; Junli Zhang; Dale E Bredesen
Journal:  J Neurochem       Date:  2007-11-06       Impact factor: 5.372

Review 8.  An old method facing a new challenge: re-visiting housekeeping proteins as internal reference control for neuroscience research.

Authors:  Rena Li; Yong Shen
Journal:  Life Sci       Date:  2013-02-27       Impact factor: 5.037

9.  How age impairs the response of the neuromuscular junction to nerve transection and repair: An experimental study in rats.

Authors:  Peter J Apel; Timothy Alton; Casey Northam; Jianjun Ma; Michael Callahan; William E Sonntag; Zhongyu Li
Journal:  J Orthop Res       Date:  2009-03       Impact factor: 3.494

10.  Effect of Age, Estrogen Status, and Late-Life GPER Activation on Cardiac Structure and Function in the Fischer344×Brown Norway Female Rat.

Authors:  Allan K Alencar; Jaqueline S da Silva; Marina Lin; Ananssa M Silva; Xuming Sun; Carlos M Ferrario; Cheping Cheng; Roberto T Sudo; Gisele Zapata-Sudo; Hao Wang; Leanne Groban
Journal:  J Gerontol A Biol Sci Med Sci       Date:  2016-03-22       Impact factor: 6.053
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.