The fractionation of t-RNA on N,N'-bis(3-aminopropyl)-piperazine substituted-Sepharose.

An anion exchange agarose has been prepared by modifying sepharose 6B with N,N'-bis (-3-aminopropyl) piperazine. This material (BAPP-Sepharose) has been used for the fractionation of t-RNA from E.coli by column chromatography. The results obtained with gram quantities of crude t-RNA at pH 4.6 and pH 8.0 as measured by the elution patterns of alanyl, arginyl, aspartyl, leucyl, lysyl, methionyl, phenylalanyl, prolyl, seryl, tyrosyl, and valyl t-RNA are described.