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Literature DB >> 10793721

The effect of bisulfite modification on the template activity of DNA for DNA polymerase I.

Abstract

Cytosine residues of poly(C) and heat-denatured calf thymus DNA were transformed into 5,6-dihydrouracil-6-sulfonate (U(SO(-) (3))) residues by treatment with bisulfite. The poly(U(SO(-) (3))(2), C(3)) and poly(U(SO(-) (3))(9), C(1)) prepared did not form inter-base binding with either poly(A) or poly(I) as judged by the absence of hypochromicity in ultraviolet absorbance. U(SO(-) (3)) residues in the DNA inactivated it to serve as template for E.coli DNA polymerase I, while the template activity was restored by conversion of the U(SO(-) (3)) residues into U.

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Year: 1974 PMID： 10793721 PMCID： PMC343392 DOI： 10.1093/nar/1.7.889

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

17 in total

2. Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.

Authors: D Shortle; D Nathans
Journal: Proc Natl Acad Sci U S A Date: 1978-05 Impact factor: 11.205

3. A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.

Authors: B Wasylyk; P Chambon
Journal: Nucleic Acids Res Date: 1981-04-24 Impact factor: 16.971

7. Amino acid substitutions within the matrix protein of type D retroviruses affect assembly, transport and membrane association of a capsid.

Authors: S S Rhee; E Hunter
Journal: EMBO J Date: 1991-03 Impact factor: 11.598

8. A rapid and efficient method for region- and strand-specific mutagenesis of cloned DNA.

Authors: R D Everett; P Chambon
Journal: EMBO J Date: 1982 Impact factor: 11.598

9. A polymerase engineered for bisulfite sequencing.

Authors: Doug Millar; Yonka Christova; Philipp Holliger
Journal: Nucleic Acids Res Date: 2015-08-13 Impact factor: 16.971

Review 10. Discovery of bisulfite-mediated cytosine conversion to uracil, the key reaction for DNA methylation analysis--a personal account.

Authors: Hikoya Hayatsu
Journal: Proc Jpn Acad Ser B Phys Biol Sci Date: 2008 Impact factor: 3.493

10 in total

The effect of bisulfite modification on the template activity of DNA for DNA polymerase I.

1. Modification of polyuridylic acid by bisulfite: effect on double helix formation and coding properties.

2. Concentration effect of bisulfite on the inactivation of transforming activity of DNA.

3. The mutagenic specificity of sodium bisulfite.

4. Bisulfite mutagenesis in bacteriophage T4.

5. The addition of sodium bisulfite to uracil and to cytosine.

6. The mutagenic action of sodium bisulfite.

7. Reaction of sodium bisulfite with uracil, cytosine, and their derivatives.

8. The cleavage of DNA by the oxygen-dependent reaction of bisulfite.

9. [Demonstration of the mutagenic action of sodium sulfite on yeast].

10. Cleavage of the glycosidic linkage of pyrimidine ribonucleosides by the bisulfite-oxygen system.

1. Improved PCR-BSP assay for multiplex methylation pattern analysis in minimal amount of DNA.

2. Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.

3. A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.

4. Mutation causing premature termination of the polyoma virus medium T antigen blocks cell transformation.

5. Segment-specific mutagenesis: extensive mutagenesis of a lac promoter/operator element.

6. Recognition of a cytosine base lesion by a human damage-specific DNA binding protein.

7. Amino acid substitutions within the matrix protein of type D retroviruses affect assembly, transport and membrane association of a capsid.

8. A rapid and efficient method for region- and strand-specific mutagenesis of cloned DNA.

9. A polymerase engineered for bisulfite sequencing.

Review 10. Discovery of bisulfite-mediated cytosine conversion to uracil, the key reaction for DNA methylation analysis--a personal account.