Literature DB >> 10793669

Fingerprinting nonradioactive ribonucleic acid with the aid of polynucleotide phosphorylase.

K S Szeto, D Söll.   

Abstract

We describe a method for obtaining radioactive fingerprints from nonradioactive ribonucleic acid. Fragments derived by T1 ribonuclease digestion of RNA are dephosphorylated with bacterial alkaline phosphatase. When these fragments are used as primers for the reaction of primer dependent polynucleotide phosphorylase with [alpha-(32)P]GDP in the presence of T1 ribonuclease the 3'-hydroxyl group of each fragment becomes phosphorylated. The degree of phosphorylation is reasonably uniform. The method has been applied to T1 ribonuclease digests of Escherichia coli tRNA(Met) (f); the oligonucleotides were further analyzed by spleen phosphodiesterase digestion. In a similar manner fingerprints of pancreatic ribonuclease digests of RNA can be obtained, when [alpha-(32)P]UDP, polynucleotide phosphorylase and pancreatic ribonuclease are used.

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Year:  1974        PMID： 10793669      PMCID： PMC343333          DOI： 10.1093/nar/1.1.171

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  Oligonucleotides as primers for polynucleotide phosphorylase.

Authors:  M F SINGER; L A HEPPEL; R J HILMOE
Journal:  J Biol Chem       Date:  1960-03       Impact factor: 5.157

2.  Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs.

Authors:  M Simsek; J Ziegenmeyer; J Heckman; U L Rajbhandary
Journal:  Proc Natl Acad Sci U S A       Date:  1973-04       Impact factor: 11.205

Review 3.  Structure and function of phage RNA.

Authors:  C Weissmann; M A Billeter; H M Goodman; J Hindley; H Weber
Journal:  Annu Rev Biochem       Date:  1973       Impact factor: 23.643

4.  The nucleotide sequence of N-formyl-methionyl-transfer RNA. Products of complete digestion with ribonuclease T-1 and pancreatic ribonuclease and derivation of their sequences.

Authors:  S K Dube; K A Marcker; B F Clark; S Cory
Journal:  Eur J Biochem       Date:  1969-03

5.  Synthesis of trinucleoside diphosphates with polynucleotide phosphorylase.

Authors:  P Leder; M F Singer; R L Brimacombe
Journal:  Biochemistry       Date:  1965-08       Impact factor: 3.162

6.  New approach to the synthesis of polyribonucleotides of defined sequence.

Authors:  J K Mackey; P T Gilham
Journal:  Nature       Date:  1971-10-22       Impact factor: 49.962

7.  Nucleotide sequence of N-formyl-methionyl-transfer RNA.

Authors:  S K Dube; K A Marcker; B F Clark; S Cory
Journal:  Nature       Date:  1968-04-20       Impact factor: 49.962

8.  Structure of transfer RNA. Evidence for interaction between two non-adjacent nucleotide residues in tRNA from Escherichia coli.

Authors:  M Yaniv; A Favre; B G Barrell
Journal:  Nature       Date:  1969-09-27       Impact factor: 49.962

9.  Use of polynucleotide kinase in fingerprinting non-radioactive nucleic acids.

Authors:  M Székely; F Sanger
Journal:  J Mol Biol       Date:  1969-08-14       Impact factor: 5.469

10.  A two-dimensional fractionation procedure for radioactive nucleotides.

Authors:  F Sanger; G G Brownlee; B G Barrell
Journal:  J Mol Biol       Date:  1965-09       Impact factor: 5.469
  10 in total
  7 in total

1.  Differences in the secondary structures of native and denatured yeast leucine transfer ribonucleic acid.

Authors:  E R Hawkins; S H Chang
Journal:  Nucleic Acids Res       Date:  1974-11       Impact factor: 16.971

2.  Tritium sequence analysis of oligoribonucleotides: a combination of post-labeling and thin-layer chromatographic techniques for the analysis of partial snake venom phosphodiesterase digests.

Authors:  M Sivarajan; R C Gupta; L L Chia; E Randerath; K Randerath
Journal:  Nucleic Acids Res       Date:  1974-10       Impact factor: 16.971

3.  A rapid method for preparation of calf spleen exonuclease.

Authors:  S Silverman; D Söll
Journal:  Nucleic Acids Res       Date:  1977-10       Impact factor: 16.971

4.  Use of specific endonuclease cleavage in RNA sequencing.

Authors:  R C Gupta; K Randerath
Journal:  Nucleic Acids Res       Date:  1977-06       Impact factor: 16.971

5.  A double-labeling procedure for sequence analysis of picomole amounts of nonradioactive RNA fragments.

Authors:  R C Gupta; E Randerath; K Randerath
Journal:  Nucleic Acids Res       Date:  1976-11       Impact factor: 16.971

6.  Sequence studies of nonradioactive Mycoplasma tRNA Phe with the aid of polynucleotide phosphorylase and polynucleotide kinase.

Authors:  K S Szeto; D Soll
Journal:  Nucleic Acids Res       Date:  1974-12       Impact factor: 16.971

7.  Sequence analysis of nonradioactive RNA fragments by periodate-phosphatase digestion and chemical tritium labeling: characterization of large oligonucleotides and oligonucleotides containing modified nucleosides.

Authors:  K Randerath; E Randerath; L S Chia; R C Gupta; M Sivarajan
Journal:  Nucleic Acids Res       Date:  1974-09       Impact factor: 16.971
  7 in total

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