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Literature DB >> 10793666

The solubility of calf thymus chromatin in sodium chloride.

Abstract

The solubility of calf thymus chromatin and chromatin depleted of F1-histone has been examined under various conditions in sodium chloride. F1-depleted DNH was more soluble than native DNH at low concentrations but this difference became small at high concentrations (1mg/ml). Both exhibited minimum solubility in 0.15M -NaCl. The effect of pH and of maleylation of the mino acid side chains on the solubility implied that electrostatic interactions dominated the precipitation reaction. Urea had no effect on the solubility of either complex. N.m.r. studies showed that the chromatin behaved as a rigid complex at all salt concentrations less than 0.6 molar.

Entities: Chemical Species

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Year: 1974 PMID： 10793666 PMCID： PMC343330 DOI： 10.1093/nar/1.1.129

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

12 in total

1. Simple computer-aided approach for the analyses of the nuclear-magnetic-resonance spectra of histones. Fractions F1, Fsa1, F2B, cleaved halves of F2B and F2B-DNA.

Authors: E M Bradbury; H W Rattle
Journal: Eur J Biochem Date: 1972-05-23

2. Studies on the role of histones in the structure of chromatin.

Authors: J E Smart; J Bonner
Journal: J Mol Biol Date: 1971-06-28 Impact factor: 5.469

3. Modification of chromatin with acetic anhydride.

Authors: R T Simpson
Journal: Biochemistry Date: 1971-11-23 Impact factor: 3.162

4. The physical state of nucleohistone under physiological ionic strength. The effect of interaction with free nucleic acids.

Authors: R H Jensen; R Chalkley
Journal: Biochemistry Date: 1968-12 Impact factor: 3.162

The solubility of calf thymus chromatin in sodium chloride.

1. Simple computer-aided approach for the analyses of the nuclear-magnetic-resonance spectra of histones. Fractions F1, Fsa1, F2B, cleaved halves of F2B and F2B-DNA.

2. Studies on the role of histones in the structure of chromatin.

3. Modification of chromatin with acetic anhydride.

4. The physical state of nucleohistone under physiological ionic strength. The effect of interaction with free nucleic acids.

5. The partial dissociation of nucleohistone by salts. Circular dichroism and denaturation studies.

6. The partial dissociation of nucleohistone by salts. Hydrodynamic and denaturation studies.

7. High resolution acrylamide gel electrophoresis of histones.

8. The use of maleic anhydride for the reversible blocking of amino groups in polypeptide chains.

9. The role of histones in the maintenance of chromatin structure.

10. Nitration of the tyrosine in histone F1 in salt solutions and in F1-polyanion complexes.

1. Histone H1--DNA interaction. On the mechanism of DNA strands crosslinking by histone H1.