Literature DB >> 10793660

Influence of template inactivators on the binding of DNA polymerase to DNA.

W E Müller, J Obermeier, A Totsuka, R K Zahn.   

Abstract

The agents daunomycin, ethidium bromide, distamycin A and cytochrome c inhibit DNA dependent DNA polymerase I (E. coli) reaction competitively to DNA. The influence of these template inactivators on the binding of DNA polymerase to native as well as denatured DNA has been determined by affinity chromatography. Cytochrome c blocks the binding of the enzyme to double-stranded and to single-stranded DNA Sepharose. In contrast to these results daunomycin, ethidium bromide or distamycin A reduce the binding affinity only with denatured DNA Sepharose as matrix. These data are discussed with respect to the modification by template inactivators of the affinity of DNA to the different binding sites of the DNA polymerase.

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Year:  1974        PMID： 10793660      PMCID： PMC343324          DOI： 10.1093/nar/1.1.63

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

1.  ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.

Authors:  C C RICHARDSON; C L SCHILDKRAUT; H V APOSHIAN; A KORNBERG
Journal:  J Biol Chem       Date:  1964-01       Impact factor: 5.157

2.  [A preservation and preparation process for desoxyribonucleic acids and their raw materials].

Authors:  R K ZAHN; E TIESLER; A K KLEINSCHMIDT; D LANG
Journal:  Biochem Z       Date:  1962

3.  The role of deoxyribonucleic acid in ribonucleic acid synthesis. III. The inhibition of the enzymatic synthesis of ribonucleic acid and deoxyribonucleic acid by actinomycin D and proflavin.

Authors:  J HURWITZ; J J FURTH; M MALAMY; M ALEXANDER
Journal:  Proc Natl Acad Sci U S A       Date:  1962-07-15       Impact factor: 11.205

4.  The amino acid composition of cytochrome c from horse heart.

Authors:  G LEAF; N E GILLIES; R PIRRIE
Journal:  Biochem J       Date:  1958-08       Impact factor: 3.857

5.  An improved method for the insolubilization of DNA for affinity chromatography.

Authors:  D Rickwood
Journal:  Biochim Biophys Acta       Date:  1972-04-26

6.  Interaction of daunomycin and its derivatives with DNA.

Authors:  F Zunino; R Gambetta; A Di Marco; A Zaccara
Journal:  Biochim Biophys Acta       Date:  1972-09-14

7.  Binding of analogues of the antibiotics distamycin A and netropsin to native DNA. Effect of chomophore systems and basic residues of the oligopeptides on thermal stability, conformation and template activity of the DNA complexes.

Authors:  C Zimmer; G Luck; H Thrum; C Pitra
Journal:  Eur J Biochem       Date:  1972-03-15

8.  Affinity chromatography of DNA-binding enzymes on single-stranded DNA-agarose columns.

Authors:  H Schaller; C Nüsslein; F J Bonhoeffer; C Kurz; I Nietzschmann
Journal:  Eur J Biochem       Date:  1972-04-24

9.  Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.

Authors:  T M Jovin; P T Englund; L L Bertsch
Journal:  J Biol Chem       Date:  1969-06-10       Impact factor: 5.157

Review 10.  Use of ethidium bromide for separation and determination of nucleic acids of various conformational forms and measurement of their associated enzymes.

Authors:  J B Le Pecq
Journal:  Methods Biochem Anal       Date:  1971
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  1 in total

1.  Magnetic isotope and magnetic field effects on the DNA synthesis.

Authors:  Anatoly L Buchachenko; Alexei P Orlov; Dmitry A Kuznetsov; Natalia N Breslavskaya
Journal:  Nucleic Acids Res       Date:  2013-07-13       Impact factor: 16.971
  1 in total

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