Literature DB >> 10790143

Direct PCR of Cryptococcus neoformans MATalpha and MATa pheromones to determine mating type, ploidy, and variety: a tool for epidemiological and molecular pathogenesis studies.

S Chaturvedi1, B Rodeghier, J Fan, C M McClelland, B L Wickes, V Chaturvedi.   

Abstract

Cryptococcus neoformans MATalpha and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry.

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Year:  2000        PMID： 10790143      PMCID： PMC86654     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Rapid assessment of S. cerevisiae mating type by PCR.

Authors:  C Huxley; E D Green; I Dunham
Journal:  Trends Genet       Date:  1990-08       Impact factor: 11.639

2.  Determination of ploidy in Cryptococcus neoformans by flow cytometry.

Authors:  R Tanaka; H Taguchi; K Takeo; M Miyaji; K Nishimura
Journal:  J Med Vet Mycol       Date:  1996 Sep-Oct

3.  Variance of ploidy in Candida albicans.

Authors:  T Suzuki; S Nishibayashi; T Kuroiwa; T Kanbe; K Tanaka
Journal:  J Bacteriol       Date:  1982-11       Impact factor: 3.490

4.  Presence of alpha and a mating types in environmental and clinical collections of Cryptococcus neoformans var. gattii strains from Australia.

Authors:  C L Halliday; T Bui; M Krockenberger; R Malik; D H Ellis; D A Carter
Journal:  J Clin Microbiol       Date:  1999-09       Impact factor: 5.948

Review 5.  On the origins of congenic MATalpha and MATa strains of the pathogenic yeast Cryptococcus neoformans.

Authors:  J Heitman; B Allen; J A Alspaugh; K J Kwon-Chung
Journal:  Fungal Genet Biol       Date:  1999-10       Impact factor: 3.495

6.  Cryptococcus neoformans var. grubii: separate varietal status for Cryptococcus neoformans serotype A isolates.

Authors:  S P Franzot; I F Salkin; A Casadevall
Journal:  J Clin Microbiol       Date:  1999-03       Impact factor: 5.948

7.  The alpha-mating type locus of Cryptococcus neoformans contains a peptide pheromone gene.

Authors:  T D Moore; J C Edman
Journal:  Mol Cell Biol       Date:  1993-03       Impact factor: 4.272

8.  Evaluation of a new method for identification of Cryptococcus neoformans which uses serologic tests aided by selected biological tests.

Authors:  K Kabasawa; H Itagaki; R Ikeda; T Shinoda; K Kagaya; Y Fukazawa
Journal:  J Clin Microbiol       Date:  1991-12       Impact factor: 5.948
  8 in total
  27 in total

1.  Mapping of the Cryptococcus neoformans MATalpha locus: presence of mating type-specific mitogen-activated protein kinase cascade homologs.

Authors:  M Karos; Y C Chang; C M McClelland; D L Clarke; J Fu; B L Wickes; K J Kwon-Chung
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

2.  Cryptococcus neoformans shows a remarkable genotypic diversity in Brazil.

Authors:  M T Barreto de Oliveira; T Boekhout; B Theelen; F Hagen; F A Baroni; M S Lazera; K B Lengeler; J Heitman; I N G Rivera; C R Paula
Journal:  J Clin Microbiol       Date:  2004-03       Impact factor: 5.948

3.  Refractory and/or Relapsing Cryptococcosis Associated with Acquired Immune Deficiency Syndrome: Clinical Features, Genotype, and Virulence Factors of Cryptococcus spp. Isolates.

Authors:  Erika Nascimento; Lucia H Vitali; Ludmilla Tonani; Marcia R Von Zeska Kress; Osvaldo M Takayanagui; Roberto Martinez
Journal:  Am J Trop Med Hyg       Date:  2016-02-29       Impact factor: 2.345

4.  PCR-restriction fragment length polymorphism analysis of the phospholipase B (PLB1) gene for subtyping of Cryptococcus neoformans isolates.

Authors:  G Nicolas Latouche; Matthew Huynh; Tania C Sorrell; Wieland Meyer
Journal:  Appl Environ Microbiol       Date:  2003-04       Impact factor: 4.792

5.  Geographic distribution of mating type alleles of Cryptococcus neoformans in four areas of the United States.

Authors:  Zhun Yan; Xiaogang Li; Jianping Xu
Journal:  J Clin Microbiol       Date:  2002-03       Impact factor: 5.948

6.  Origin of Cryptococcus neoformans var. neoformans diploid strains.

Authors:  M Cogliati; M C Esposto; D L Clarke; B L Wickes; M A Viviani
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

7.  Transcription factor STE12alpha has distinct roles in morphogenesis, virulence, and ecological fitness of the primary pathogenic yeast Cryptococcus gattii.

Authors:  Ping Ren; Deborah J Springer; Melissa J Behr; William A Samsonoff; Sudha Chaturvedi; Vishnu Chaturvedi
Journal:  Eukaryot Cell       Date:  2006-07

8.  First environmental isolations of Cryptococcus neoformans and Cryptococcus gattii in Tunisia and review of published studies on environmental isolations in Africa.

Authors:  F Mseddi; A Sellami; M A Jarboui; H Sellami; F Makni; A Ayadi
Journal:  Mycopathologia       Date:  2011-01-01       Impact factor: 2.574

9.  Cryptococcus neoformans Isolated from Passerine and Psittacine bird excreta in the state of Paraná, Brazil.

Authors:  Camile Lugarini; Cristine Souza Goebel; Larissa Anuska Zeni Condas; Marisol Dominguez Muro; Marconi Rodrigues de Farias; Fabiano Montiani Ferreira; Marilene H Vainstein
Journal:  Mycopathologia       Date:  2008-05-06       Impact factor: 2.574

10.  Molecular analysis of Cryptococcus neoformans mitochondrial cytochrome b gene sequences.

Authors:  Swarajit Kumar Biswas; Li Wang; Koji Yokoyama; Kazuko Nishimura
Journal:  J Clin Microbiol       Date:  2003-12       Impact factor: 5.948
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