Literature DB >> 10790134

Sensitive method for detection of human herpesviruses 6 and 7 in saliva collected in field studies.

D M Zerr1, M L Huang, L Corey, M Erickson, H L Parker, L M Frenkel.   

Abstract

To facilitate studies of the epidemiology and natural history of human herpesviruses 6 and 7 in infants, a practical method for collecting and quantifying the DNA of these viruses was developed. Saliva was collected using small strips of filter paper, and virus was detected using a real-time quantitative fluorescent-probe PCR assay. The sensitivity and specificity of this method even after prolonged drying of the specimens compared favorably to those of our traditional method of collecting and assaying saliva.

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Year:  2000        PMID： 10790134      PMCID： PMC86642     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

1.  Measurement of human herpesvirus 7 load in peripheral blood and saliva of healthy subjects by quantitative polymerase chain reaction.

Authors:  I M Kidd; D A Clark; M Ait-Khaled; P D Griffiths; V C Emery
Journal:  J Infect Dis       Date:  1996-08       Impact factor: 5.226

2.  Real time quantitative PCR.

Authors:  C A Heid; J Stevens; K J Livak; P M Williams
Journal:  Genome Res       Date:  1996-10       Impact factor: 9.043

3.  Herpes simplex virus type 2 detection by culture and polymerase chain reaction and relationship to genital symptoms and cervical antibody status during the third trimester of pregnancy.

Authors:  K A Boggess; D H Watts; A C Hobson; R L Ashley; Z A Brown; L Corey
Journal:  Am J Obstet Gynecol       Date:  1997-02       Impact factor: 8.661

4.  Detection of human herpesvirus 6 DNA in throat swabs by polymerase chain reaction.

Authors:  S Kido; K Kondo; T Kondo; T Morishima; M Takahashi; K Yamanishi
Journal:  J Med Virol       Date:  1990-11       Impact factor: 2.327

5.  Presence of human herpesvirus 6 variants A and B in saliva and peripheral blood mononuclear cells of healthy adults.

Authors:  S W Aberle; C W Mandl; C Kunz; T Popow-Kraupp
Journal:  J Clin Microbiol       Date:  1996-12       Impact factor: 5.948

6.  Human herpesvirus 6 infections after bone marrow transplantation: clinical and virologic manifestations.

Authors:  R W Cone; M L Huang; L Corey; J Zeh; R Ashley; R Bowden
Journal:  J Infect Dis       Date:  1999-02       Impact factor: 5.226

7.  Human herpesvirus 6 DNA in peripheral blood cells and saliva from immunocompetent individuals.

Authors:  R W Cone; M L Huang; R Ashley; L Corey
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948

8.  Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.

Authors:  P M Holland; R D Abramson; R Watson; D H Gelfand
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-15       Impact factor: 11.205

9.  Frequent isolation of HHV-6 from saliva and high seroprevalence of the virus in the population.

Authors:  J A Levy; F Ferro; D Greenspan; E T Lennette
Journal:  Lancet       Date:  1990-05-05       Impact factor: 79.321

10.  Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples.

Authors:  A J Ryncarz; J Goddard; A Wald; M L Huang; B Roizman; L Corey
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948
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  23 in total

1.  Real-time detection of gene promoter activity: quantitation of toxin gene transcription.

Authors:  K Jeyaseelan; D Ma; A Armugam
Journal:  Nucleic Acids Res       Date:  2001-06-15       Impact factor: 16.971

Review 2.  Real-time PCR in virology.

Authors:  Ian M Mackay; Katherine E Arden; Andreas Nitsche
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

3.  Chromatography paper strip method for collection, transportation, and storage of rotavirus RNA in stool samples.

Authors:  Mustafizur Rahman; Truus Goegebuer; Karolien De Leener; Piet Maes; Jelle Matthijnssens; Goutam Podder; Tasnim Azim; Marc Van Ranst
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948

4.  Real-time PCR assay using specimens on filter disks as a template for detection of cytomegalovirus in urine.

Authors:  Naoki Nozawa; Shin Koyano; Yumiko Yamamoto; Yuhki Inami; Ichiro Kurane; Naoki Inoue
Journal:  J Clin Microbiol       Date:  2007-02-07       Impact factor: 5.948

5.  Human Bocavirus 1 Primary Infection and Shedding in Infants.

Authors:  Emily T Martin; Jane Kuypers; John P McRoberts; Janet A Englund; Danielle M Zerr
Journal:  J Infect Dis       Date:  2015-01-28       Impact factor: 5.226

6.  High prevalence of multiple human herpesviruses in saliva from human immunodeficiency virus-infected persons in the era of highly active antiretroviral therapy.

Authors:  Craig S Miller; Joseph R Berger; Yunanan Mootoor; Sergei A Avdiushko; Hua Zhu; Richard J Kryscio
Journal:  J Clin Microbiol       Date:  2006-07       Impact factor: 5.948

7.  Real time RT-PCR quantification and Northern analysis of cerato-ulmin ( CU) gene transcription in different strains of the phytopathogens Ophiostoma ulmi and O. novo-ulmi.

Authors:  Y Tadesse; L Bernier; W E Hintz; P A Horgen
Journal:  Mol Genet Genomics       Date:  2003-07-24       Impact factor: 3.291

8.  Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.

Authors:  Meei-Li Huang; Long Nguy; James Ferrenberg; Michael Boeckh; Anne Cent; Lawrence Corey
Journal:  Diagn Microbiol Infect Dis       Date:  2008-08-15       Impact factor: 2.803

9.  Cytomegalovirus and Epstein-Barr virus in breast milk are associated with HIV-1 shedding but not with mastitis.

Authors:  Soren Gantt; Jacquelyn Carlsson; Avinash K Shetty; Kristy D Seidel; Xuan Qin; Junior Mutsvangwa; Georgina Musingwini; Godfrey Woelk; Lynn S Zijenah; David A Katzenstein; Lisa M Frenkel
Journal:  AIDS       Date:  2008-07-31       Impact factor: 4.177

10.  PCR-based assay using occult blood detection cards for detection of diarrheagenic Escherichia coli in specimens from U.S. travelers to Mexico with acute diarrhea.

Authors:  Kevin A Grimes; Jamal A Mohamed; Herbert L Dupont; Ranjit S Padda; Zhi-Dong Jiang; Jose Flores; Jaime Belkind-Gerson; Francisco G Martinez-Sandoval; Pablo C Okhuysen
Journal:  J Clin Microbiol       Date:  2008-05-14       Impact factor: 5.948
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