Literature DB >> 10788413

Fortified sera and their use in environmental virology.

J L Hoyt1, A B Margolin.   

Abstract

Four commercially available fortified sera were compared to fetal bovine serum (FBS) with regard to their ability to maintain or increase the sensitivity of the Buffalo green monkey (BGM) kidney cell line to viral infection. Nine virus strains and five wastewater samples were used. Fortified sera were comparable to FBS for the enumeration of some viruses by the plaque method and for the detection of virus in wastewater by the most-probable-number assay.

Entities:  

Mesh：

Substances：

Year:  2000        PMID： 10788413      PMCID： PMC101486          DOI： 10.1128/AEM.66.5.2259-2262.2000

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  4 in total

1.  Optimization of the BGM cell line culture and viral assay procedures for monitoring viruses in the environment.

Authors:  D R Dahling; B A Wright
Journal:  Appl Environ Microbiol       Date:  1986-04       Impact factor: 4.792

2.  Reverse transcription PCR to detect enteroviruses in surface water.

Authors:  M Gilgen; B Wegmüller; P Burkhalter; H P Bühler; U Müller; J Lüthy; U Candrian
Journal:  Appl Environ Microbiol       Date:  1995-04       Impact factor: 4.792

3.  Comparison of fortified calf serum, serum substitutes and fetal calf serum with or without extenders for propagation of cell cultures for virus plaque assays.

Authors:  D R Dahling; B A Wright
Journal:  J Virol Methods       Date:  1990-03       Impact factor: 2.014

4.  Use of serum substitutes for the growth of four cell lines commonly used for virus isolation.

Authors:  C A Gleaves; D H Rice; J D Meyers
Journal:  J Virol Methods       Date:  1990-05       Impact factor: 2.014
  4 in total
  1 in total

1.  Detection of poliovirus by ICC/qPCR in concentrated water samples has greater sensitivity and is less costly using BGM cells in suspension as compared to monolayers.

Authors:  Helene B Balkin; Aaron B Margolin
Journal:  Virol J       Date:  2010-10-25       Impact factor: 4.099
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.