Literature DB >> 10786879

The current state of two-dimensional electrophoresis with immobilized pH gradients.

A Görg1, C Obermaier, G Boguth, A Harder, B Scheibe, R Wildgruber, W Weiss.   

Abstract

The original protocol of two-dimensional electrophoresis with immobilized pH gradient (IPG-Dalt; Gorg et al., Electrophoresis 1988, 9, 531-546) is updated. Merits and limits of different methods for sample solubilization, sample application (by cup-loading or ingel rehydration) with respect to the pH interval used for IPG-isoelectric focusing are critically discussed. Guidelines for running conditions of analytical and micropreparative IPG-Dalt, using wide IPGs up to pH 12 for overview patterns, or narrow IPGs for zoom-in gels for optimum resolution and detection of minor components, are stated. Results with extended separation distances as well as automated procedures are demonstrated, and a comparison between protein detection by silver staining and fluorescent dyes is given. A brief trouble shooting guide is also included.

Entities:  

Mesh：

Substances：

Year:  2000        PMID： 10786879     DOI： 10.1002/(SICI)1522-2683(20000401)21:6<1037::AID-ELPS1037>3.0.CO;2-V

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  256 in total

1.  Functional proteomics of transforming growth factor-beta1-stimulated Mv1Lu epithelial cells: Rad51 as a target of TGFbeta1-dependent regulation of DNA repair.

Authors:  Takashi Kanamoto; Ulf Hellman; Carl-Henrik Heldin; Serhiy Souchelnytskyi
Journal:  EMBO J       Date:  2002-03-01       Impact factor: 11.598

Review 2.  Molecular biologist's guide to proteomics.

Authors:  Paul R Graves; Timothy A J Haystead
Journal:  Microbiol Mol Biol Rev       Date:  2002-03       Impact factor: 11.056

3.  Re-annotating the Mycoplasma pneumoniae genome sequence: adding value, function and reading frames.

Authors:  T Dandekar; M Huynen; J T Regula; B Ueberle; C U Zimmermann; M A Andrade; T Doerks; L Sánchez-Pulido; B Snel; M Suyama; Y P Yuan; R Herrmann; P Bork
Journal:  Nucleic Acids Res       Date:  2000-09-01       Impact factor: 16.971

4.  High-resolution dissection of phagosome maturation reveals distinct membrane trafficking phases.

Authors:  Daniel Gotthardt; Hans Jörg Warnatz; Oliver Henschel; Franz Brückert; Michael Schleicher; Thierry Soldati
Journal:  Mol Biol Cell       Date:  2002-10       Impact factor: 4.138

5.  Hydrophobic protein in colorectal cancer in relation to tumor stages and grades.

Authors:  Lay-Chin Yeoh; Chee-Keat Loh; Boon-Hui Gooi; Manjit Singh; Lay-Harn Gam
Journal:  World J Gastroenterol       Date:  2010-06-14       Impact factor: 5.742

Review 6.  Proteomics as a tool to monitor plant-microbe endosymbioses in the rhizosphere.

Authors:  G Bestel-Corre; E Dumas-Gaudot; S Gianinazzi
Journal:  Mycorrhiza       Date:  2003-11-19       Impact factor: 3.387

7.  Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures.

Authors:  J Weiner; C-U Zimmerman; H W H Göhlmann; R Herrmann
Journal:  Nucleic Acids Res       Date:  2003-11-01       Impact factor: 16.971

Review 8.  The application of 2D gel-based proteomics methods to the study of breast cancer.

Authors:  Robert C Stein; Marketa J Zvelebil
Journal:  J Mammary Gland Biol Neoplasia       Date:  2002-10       Impact factor: 2.673

Review 9.  Proteomics for protein expression profiling in neuroscience.

Authors:  Willard M Freeman; Scott E Hemby
Journal:  Neurochem Res       Date:  2004-06       Impact factor: 3.996

10.  Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phenol extraction for crop proteomic analysis.

Authors:  Xiaolin Wu; Erhui Xiong; Wei Wang; Monica Scali; Mauro Cresti
Journal:  Nat Protoc       Date:  2014-01-16       Impact factor: 13.491
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.