Literature DB >> 10781573

Positioning of region 4 of the Escherichia coli RNA polymerase sigma(70) subunit by a transcription activator.

J A Bown1, A Kolb, C F Meares, A Ishihama, S D Minchin, S J Busby.   

Abstract

A DNA cleavage reagent, specifically tethered to residue 581 of the Escherichia coli RNA polymerase sigma(70) subunit, has been used to investigate the location of sigma(70) region 4 in different complexes at the galp(1) promoter and the effect of the cyclic AMP receptor protein. The positions of DNA cleavage by the reagent are not affected by the cyclic AMP receptor protein. We conclude that transcription activation at the galp(1) promoter by the cyclic AMP receptor protein does not involve major conformation changes in or repositioning of sigma(70) region 4.

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Year:  2000        PMID： 10781573      PMCID： PMC102013          DOI： 10.1128/JB.182.10.2982-2984.2000

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  15 in total

Review 1.  Positive activation of gene expression.

Authors:  V A Rhodius; S J Busby
Journal:  Curr Opin Microbiol       Date:  1998-04       Impact factor: 7.934

2.  Dual control for transcription of the galactose operon by cyclic AMP and its receptor protein at two interspersed promoters.

Authors:  R E Musso; R Di Lauro; S Adhya; B de Crombrugghe
Journal:  Cell       Date:  1977-11       Impact factor: 41.582

Review 3.  Transcription activation at class II CAP-dependent promoters.

Authors:  S Busby; R H Ebright
Journal:  Mol Microbiol       Date:  1997-03       Impact factor: 3.501

4.  Transcription activation at class II CRP-dependent promoters: the role of different activating regions.

Authors:  V A Rhodius; D M West; C L Webster; S J Busby; N J Savery
Journal:  Nucleic Acids Res       Date:  1997-01-15       Impact factor: 16.971

5.  Orientation of functional activating regions in the Escherichia coli CRP protein during transcription activation at class II promoters.

Authors:  R M Williams; V A Rhodius; A I Bell; A Kolb; S J Busby
Journal:  Nucleic Acids Res       Date:  1996-03-15       Impact factor: 16.971

Review 6.  Promoter structure, promoter recognition, and transcription activation in prokaryotes.

Authors:  S Busby; R H Ebright
Journal:  Cell       Date:  1994-12-02       Impact factor: 41.582

7.  Location of the C-terminal domain of the RNA polymerase alpha subunit in different open complexes at the Escherichia coli galactose operon regulatory region.

Authors:  T A Belyaeva; J A Bown; N Fujita; A Ishihama; S J Busby
Journal:  Nucleic Acids Res       Date:  1996-06-15       Impact factor: 16.971

8.  Scanning calorimetric study of the thermal unfolding of catabolite activator protein from Escherichia coli in the absence and presence of cyclic mononucleotides.

Authors:  L R Ghosaini; A M Brown; J M Sturtevant
Journal:  Biochemistry       Date:  1988-07-12       Impact factor: 3.162

9.  Transcription activation by cAMP receptor protein (CRP) at the Escherichia coli gal P1 promoter. Crucial role for the spacing between the CRP binding site and the -10 region.

Authors:  M Lavigne; A Kolb; H Buc
Journal:  Biochemistry       Date:  1992-10-13       Impact factor: 3.162

10.  Upstream curved sequences influence the initiation of transcription at the Escherichia coli galactose operon.

Authors:  M Lavigne; M Herbert; A Kolb; H Buc
Journal:  J Mol Biol       Date:  1992-03-20       Impact factor: 5.469
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  5 in total

1.  Interactions among CII protein, RNA polymerase and the lambda PRE promoter: contacts between RNA polymerase and the -35 region of PRE are identical in the presence and absence of CII protein.

Authors:  Michael T Marr; Jeffrey W Roberts; Susan E Brown; Matthew Klee; Gary N Gussin
Journal:  Nucleic Acids Res       Date:  2004-02-10       Impact factor: 16.971

2.  The interaction between sigma70 and the beta-flap of Escherichia coli RNA polymerase inhibits extension of nascent RNA during early elongation.

Authors:  Bryce E Nickels; Sean J Garrity; Vladimir Mekler; Leonid Minakhin; Konstantin Severinov; Richard H Ebright; Ann Hochschild
Journal:  Proc Natl Acad Sci U S A       Date:  2005-03-10       Impact factor: 11.205

3.  Promoter activation by repositioning of RNA polymerase.

Authors:  Amrita Kumar; Charles P Moran
Journal:  J Bacteriol       Date:  2008-02-22       Impact factor: 3.490

4.  Genetic evidence for a novel interaction between transcriptional activator SoxS and region 4 of the σ(70) subunit of RNA polymerase at class II SoxS-dependent promoters in Escherichia coli.

Authors:  M Ammar Zafar; Neus Sanchez-Alberola; Richard E Wolf
Journal:  J Mol Biol       Date:  2010-12-31       Impact factor: 5.469

5.  The bacteriophage T4 transcription activator MotA interacts with the far-C-terminal region of the sigma70 subunit of Escherichia coli RNA polymerase.

Authors:  Suchira Pande; Anna Makela; Simon L Dove; Bryce E Nickels; Ann Hochschild; Deborah M Hinton
Journal:  J Bacteriol       Date:  2002-07       Impact factor: 3.490
  5 in total

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