| Literature DB >> 10769757 |
L Y Wong1, V Belonogoff, V L Boyd, N M Hunkapiller, P M Casey, S N Liew, K D Lazaruk, S Baumhueter.
Abstract
Gene expression profiling using an AFLP-based technique generates a large number of gene fragments that require identification by sequencing. The DNA fragments vary in length from about 50-500 bp. Ion-pair reversed-phase HPLC can be used to purify selected double-stranded DNA fragments that represent differentially expressed genes. The gene fragments are sequenced directly after vacuum drying of the collected HPLC fractions.Mesh：
Substances：
Year: 2000 PMID： 10769757 DOI： 10.2144/00284rr07
Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993