| Literature DB >> 10769744 |
R Akada1, T Murakane, Y Nishizawa.
Abstract
A simple procedure for isolating yeast DNA suitable for use as a template for PCR amplification is described. SDS treatment alone is sufficient for extraction of chromosomal DNA from yeast cells. Cells of a yeast colony are suspended in a small volume (about 20 microL) of a 0.25% SDS solution, mixed vigorously and centrifuged. The supernatant can be directly used as a template after dilution to give an SDS concentration of less than 0.01% in the final PCR mixture.Entities:
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Year: 2000 PMID： 10769744 DOI： 10.2144/00284st02
Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993