A J Simpson1, V Wuthiekanun. 1. Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. fnajs@diamond.mahidol.ac.th
Abstract
AIM: To re-examine the previously reported in vitro interaction of insulin with Burkholderia pseudomallei, in the light of a suggestion that the interaction may have resulted from the presence of the preservative m-cresol in commercial preparations. METHODS: Broth culture studies of B pseudomallei were performed with and without the addition of m-cresol and various preparations of insulin. RESULTS: Growth of B pseudomallei was inhibited by m-cresol at the concentrations found in pharmaceutical insulin preparations, and by the insulin preparation Humulin R, but not by pure insulin. CONCLUSIONS: The results of previous experiments may have been confounded by the presence of the preservative m-cresol.
AIM: To re-examine the previously reported in vitro interaction of insulin with Burkholderia pseudomallei, in the light of a suggestion that the interaction may have resulted from the presence of the preservative m-cresol in commercial preparations. METHODS: Broth culture studies of B pseudomallei were performed with and without the addition of m-cresol and various preparations of insulin. RESULTS: Growth of B pseudomallei was inhibited by m-cresol at the concentrations found in pharmaceutical insulin preparations, and by the insulin preparation Humulin R, but not by pure insulin. CONCLUSIONS: The results of previous experiments may have been confounded by the presence of the preservative m-cresol.