Literature DB >> 10767547

Identification and recombinant expression of glyceraldehyde-3-phosphate dehydrogenase of Plasmodium falciparum.

C A Daubenberger1, F Pöltl-Frank, G Jiang, J Lipp, U Certa, G Pluschke.   

Abstract

The gene coding for the cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPDH; EC 1.2.1.12) was isolated from Plasmodium falciparum. The gene contains 1 intron and the A+T content is characteristic for the codon usage of P. falciparum. The predicted open reading frame codes for 337 amino acids (36651Da) and is 63.5% identical to the human erythrocytic GAPDH. GAPDH sequences from several field isolates of P. falciparum displayed 100% conservation. Phylogenetic analysis supports the hypothesis that dinoflagellates and Plasmodium are closely related. The protein encoded by the pfGAPDH was expressed recombinantly in Escherichia coli and exhibited enzymatic activity with NAD(+) but not with NADP(+) as cofactor. Antiserum raised against the recombinantly expressed enzyme detected specifically all developmental stages of cultured P. falciparum blood-stage parasites.

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Year:  2000        PMID： 10767547     DOI： 10.1016/s0378-1119(00)00069-x

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  6 in total

1.  A homolog of glyceraldehyde-3-phosphate dehydrogenase from Riemerella anatipestifer is an extracellular protein and exhibits biological activity.

Authors:  Ji-ye Gao; Cui-lian Ye; Li-li Zhu; Zhi-ying Tian; Zhi-bang Yang
Journal:  J Zhejiang Univ Sci B       Date:  2014-09       Impact factor: 3.066

2.  Recombinant human sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDHS) is expressed at high yield as an active homotetramer in baculovirus-infected insect cells.

Authors:  David R Lamson; Alan J House; Polina V Danshina; Jonathan Z Sexton; Khaddijatou Sanyang; Deborah A O'Brien; Li-An Yeh; Kevin P Williams
Journal:  Protein Expr Purif       Date:  2010-09-07       Impact factor: 1.650

Review 3.  The ferredoxin redox system - an essential electron distributing hub in the apicoplast of Apicomplexa.

Authors:  Ojo-Ajogu Akuh; Rubayet Elahi; Sean T Prigge; Frank Seeber
Journal:  Trends Parasitol       Date:  2022-08-20

4.  Identification and characterization of a conserved, stage-specific gene product of Plasmodium falciparum recognized by parasite growth inhibitory antibodies.

Authors:  Claudia A Daubenberger; Diana Diaz; Marija Curcic; Markus S Mueller; Tobias Spielmann; Ulrich Certa; Joachim Lipp; Gerd Pluschke
Journal:  Infect Immun       Date:  2003-04       Impact factor: 3.441

Review 5.  Exploiting unique structural and functional properties of malarial glycolytic enzymes for antimalarial drug development.

Authors:  Asrar Alam; Md Kausar Neyaz; Syed Ikramul Hasan
Journal:  Malar Res Treat       Date:  2014-12-17

6.  Evidence for the Nucleo-Apical Shuttling of a Beta-Catenin Like Plasmodium falciparum Armadillo Repeat Containing Protein.

Authors:  Pallabi Mitra; Enna Dogra Gupta; Tajali Sahar; Alok K Pandey; Poonam Dangi; K Sony Reddy; Virander Singh Chauhan; Deepak Gaur
Journal:  PLoS One       Date:  2016-02-01       Impact factor: 3.240
  6 in total

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