Literature DB >> 10767539

A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae.

A De Antoni1, D Gallwitz.   

Abstract

Gene tagging can be achieved by homologous recombination in yeast. The kan(r) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kan(r) marker repeatedly.

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Year:  2000        PMID： 10767539     DOI： 10.1016/s0378-1119(00)00083-4

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  33 in total

1.  New suppressors of THO mutations identify Thp3 (Ypr045c)-Csn12 as a protein complex involved in transcription elongation.

Authors:  Sonia Jimeno; Cristina Tous; María L García-Rubio; Michael Ranes; Cristina González-Aguilera; Antonio Marín; Andrés Aguilera
Journal:  Mol Cell Biol       Date:  2010-12-13       Impact factor: 4.272

2.  Ordered assembly of Sld3, GINS and Cdc45 is distinctly regulated by DDK and CDK for activation of replication origins.

Authors:  Hayato Yabuuchi; Yoshiki Yamada; Tomonori Uchida; Tul Sunathvanichkul; Takuro Nakagawa; Hisao Masukata
Journal:  EMBO J       Date:  2006-09-21       Impact factor: 11.598

3.  Examining protein protein interactions using endogenously tagged yeast arrays: the cross-and-capture system.

Authors:  Bernhard Suter; Michael J Fetchko; Ralph Imhof; Christopher I Graham; Ingrid Stoffel-Studer; Caroline Zbinden; Maanasa Raghavan; Lianet Lopez; Lucija Beneti; Jacqueline Hort; Jeffrey Fillingham; Jack F Greenblatt; Guri Giaever; Corey Nislow; Igor Stagljar
Journal:  Genome Res       Date:  2007-11-07       Impact factor: 9.043

4.  Activator and repressor functions of the Mot3 transcription factor in the osmostress response of Saccharomyces cerevisiae.

Authors:  Fernando Martínez-Montañés; Alessandro Rienzo; Daniel Poveda-Huertes; Amparo Pascual-Ahuir; Markus Proft
Journal:  Eukaryot Cell       Date:  2013-02-22

5.  Live-cell assays reveal selectivity and sensitivity of the multidrug response in budding yeast.

Authors:  Elena Vanacloig-Pedros; Carlos Lozano-Pérez; Benito Alarcón; Amparo Pascual-Ahuir; Markus Proft
Journal:  J Biol Chem       Date:  2019-07-11       Impact factor: 5.157

6.  A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast.

Authors:  U Gueldener; J Heinisch; G J Koehler; D Voss; J H Hegemann
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

7.  An acidic sequence of a putative yeast Golgi membrane protein binds COPII and facilitates ER export.

Authors:  C Votsmeier; D Gallwitz
Journal:  EMBO J       Date:  2001-12-03       Impact factor: 11.598

8.  Rad51 suppresses gross chromosomal rearrangement at centromere in Schizosaccharomyces pombe.

Authors:  Ken-ichi Nakamura; Aya Okamoto; Yuki Katou; Chie Yadani; Takeshi Shitanda; Chitrada Kaweeteerawat; Tatsuro S Takahashi; Takehiko Itoh; Katsuhiko Shirahige; Hisao Masukata; Takuro Nakagawa
Journal:  EMBO J       Date:  2008-10-16       Impact factor: 11.598

9.  Positive and negative roles of homologous recombination in the maintenance of genome stability in Saccharomyces cerevisiae.

Authors:  Jumpei Yoshida; Keiko Umezu; Hisaji Maki
Journal:  Genetics       Date:  2003-05       Impact factor: 4.562

10.  Short tetracysteine tags to beta-tubulin demonstrate the significance of small labels for live cell imaging.

Authors:  Martin Andresen; Rita Schmitz-Salue; Stefan Jakobs
Journal:  Mol Biol Cell       Date:  2004-10-06       Impact factor: 4.138
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