Literature DB >> 10766307

Construction of a full length infectious clone for dengue-1 virus Western Pacific,74 strain.

B Puri1, S Polo, C G Hayes, B Falgout.   

Abstract

The flavivirus dengue 1 Western Pacific,74 (DEN1 WP) virus has a positive-stranded RNA genome of 10,735 nucleotides. DEN1 WP genomic RNA was amplified into three overlapping fragments by RT-PCR. These fragments were assembled into a full-length cDNA clone in the yeast-E. coli shuttle vector pRS424, using homologous recombination in yeast. RNA produced by in vitro transcription of this clone was infectious upon electroporation into LLCMK2 cells, as shown by cytopathic effects and detection of viral antigens by indirect immunofluorescence, and by propagation of the virus released into the culture media. Biological properties of the transcript-derived virus, such as the pattern of dengue-specific protein synthesis and growth rate in LLCMK2 or C6/36 cells, resembled those of the parent DEN1 WP virus.

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Year:  2000        PMID： 10766307     DOI： 10.1023/a:1008160123754

Source DB:  PubMed          Journal:  Virus Genes        ISSN： 0920-8569            Impact factor:   2.332


  22 in total

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Authors:  W E Brandt
Journal:  J Infect Dis       Date:  1990-09       Impact factor: 5.226

2.  Complete nucleotide sequence analysis of a Western Pacific dengue-1 virus strain.

Authors:  B Puri; W Nelson; K R Porter; E A Henchal; C G Hayes
Journal:  Virus Genes       Date:  1998       Impact factor: 2.332

3.  From the World Health Organization. Current approaches to the development of dengue vaccines and related aspects of the molecular biology of flaviviruses.

Authors:  W E Brandt
Journal:  J Infect Dis       Date:  1988-05       Impact factor: 5.226

4.  Infectious RNA transcribed from stably cloned full-length cDNA of dengue type 4 virus.

Authors:  C J Lai; B T Zhao; H Hori; M Bray
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-15       Impact factor: 11.205

5.  A live attenuated dengue-1 vaccine candidate (45AZ5) passaged in primary dog kidney cell culture is attenuated and immunogenic for humans.

Authors:  R Edelman; C O Tacket; S S Wasserman; D W Vaughn; K H Eckels; D R Dubois; P L Summers; C H Hoke
Journal:  J Infect Dis       Date:  1994-12       Impact factor: 5.226

6.  Antibody-enhanced dengue virus infection in primate leukocytes.

Authors:  S B Halstead; E J O'Rourke
Journal:  Nature       Date:  1977-02-24       Impact factor: 49.962

7.  Identification of a major determinant of mouse neurovirulence of dengue virus type 2 using stably cloned genomic-length cDNA.

Authors:  R C Gualano; M J Pryor; M R Cauchi; P J Wright; A D Davidson
Journal:  J Gen Virol       Date:  1998-03       Impact factor: 3.891

Review 8.  Pathogenesis of dengue: challenges to molecular biology.

Authors:  S B Halstead
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

9.  Elimination of L-A double-stranded RNA virus of Saccharomyces cerevisiae by expression of gag and gag-pol from an L-A cDNA clone.

Authors:  R P Valle; R B Wickner
Journal:  J Virol       Date:  1993-05       Impact factor: 5.103

10.  Dengue viruses and mononuclear phagocytes. II. Identity of blood and tissue leukocytes supporting in vitro infection.

Authors:  S B Halstead; E J O'Rourke; A C Allison
Journal:  J Exp Med       Date:  1977-07-01       Impact factor: 14.307
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  25 in total

1.  Derivation and characterization of a dengue type 1 host range-restricted mutant virus that is attenuated and highly immunogenic in monkeys.

Authors:  Lewis Markoff; Xiaou Pang; Huo-shu Houng Hs; Barry Falgout; Raymond Olsen; Estella Jones; Stephanie Polo
Journal:  J Virol       Date:  2002-04       Impact factor: 5.103

2.  Complex phenotypes in mosquitoes and mice associated with neutralization escape of a Dengue virus type 1 monoclonal antibody.

Authors:  Bimmi Shrestha; S Kyle Austin; Kimberly A Dowd; Abhishek N Prasad; Soonjeon Youn; Theodore C Pierson; Daved H Fremont; Gregory D Ebel; Michael S Diamond
Journal:  Virology       Date:  2012-03-09       Impact factor: 3.616

3.  Triaryl pyrazoline compound inhibits flavivirus RNA replication.

Authors:  Francesc Puig-Basagoiti; Mark Tilgner; Brett M Forshey; Sean M Philpott; Noel G Espina; David E Wentworth; Scott J Goebel; Paul S Masters; Barry Falgout; Ping Ren; David M Ferguson; Pei-Yong Shi
Journal:  Antimicrob Agents Chemother       Date:  2006-04       Impact factor: 5.191

4.  Successful propagation of flavivirus infectious cDNAs by a novel method to reduce the cryptic bacterial promoter activity of virus genomes.

Authors:  Szu-Yuan Pu; Ren-Huang Wu; Chi-Chen Yang; Tzu-Ming Jao; Ming-Han Tsai; Jing-Chyi Wang; Hui-Mei Lin; Yu-Sheng Chao; Andrew Yueh
Journal:  J Virol       Date:  2011-01-12       Impact factor: 5.103

5.  Dengue reporter viruses reveal viral dynamics in interferon receptor-deficient mice and sensitivity to interferon effectors in vitro.

Authors:  John W Schoggins; Marcus Dorner; Michael Feulner; Naoko Imanaka; Mary Y Murphy; Alexander Ploss; Charles M Rice
Journal:  Proc Natl Acad Sci U S A       Date:  2012-08-20       Impact factor: 11.205

6.  In vitro and in vivo studies identify important features of dengue virus pr-E protein interactions.

Authors:  Aihua Zheng; Mahadevaiah Umashankar; Margaret Kielian
Journal:  PLoS Pathog       Date:  2010-10-21       Impact factor: 6.823

7.  Live attenuated tetravalent dengue virus host range vaccine is immunogenic in African green monkeys following a single vaccination.

Authors:  Caitlin M Briggs; Katherine M Smith; Amanda Piper; Emerson Huitt; Carla J Spears; Michelle Quiles; Mariana Ribeiro; Malcolm E Thomas; Dennis T Brown; Raquel Hernandez
Journal:  J Virol       Date:  2014-04-02       Impact factor: 5.103

8.  A live, attenuated dengue virus type 1 vaccine candidate with a 30-nucleotide deletion in the 3' untranslated region is highly attenuated and immunogenic in monkeys.

Authors:  Stephen S Whitehead; Barry Falgout; Kathryn A Hanley; Joseph E Blaney; Lewis Markoff; Brian R Murphy
Journal:  J Virol       Date:  2003-01       Impact factor: 5.103

9.  Development and characterization of a reverse genetic system for studying dengue virus serotype 3 strain variation and neutralization.

Authors:  William B Messer; Boyd Yount; Kari E Hacker; Eric F Donaldson; Jeremy P Huynh; Aravinda M de Silva; Ralph S Baric
Journal:  PLoS Negl Trop Dis       Date:  2012-02-28

10.  Development of Dengue virus type 2 replicons capable of prolonged expression in host cells.

Authors:  X Pang; M Zhang; A I Dayton
Journal:  BMC Microbiol       Date:  2001-08-24       Impact factor: 3.605
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