| Literature DB >> 10765985 |
R G Zhang1, X W Wang, J H Yuan, L X Guo, H Xie.
Abstract
A non-radioisotopic, quantitative TRAP-based telomerase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with conventional radioisotope based method, it was better in reproducibility and accuracy. Using this method, we found telomerase activities were absent in normal human liver cells, while detected in all of four human hepatoma cell lines (BEL-7404, SMMC-7721, QGY-7903 and HCCM) without significant differences.Entities:
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Year: 2000 PMID： 10765985 DOI： 10.1038/sj.cr.7290037
Source DB: PubMed Journal: Cell Res ISSN： 1001-0602 Impact factor: 25.617