K Gutensohn1, A Alisch, W Krueger, N Kroeger, P Kuehnl. 1. Transfusion Medicine/Transplantation Immunology, University Hospital Eppendorf, University of Hamburg, Hamburg, Germany. gutensohn@uke.uni-hamburg.de
Abstract
BACKGROUND AND OBJECTIVES: In this study we investigated whether platelet activation during apheresis results in the binding of platelets to white blood cells. MATERIAL AND METHODS: Analysis of platelet-leukocyte interaction was performed using multiparameter, three-color flow cytometry. RESULTS: Over the duration of the procedure, there was an increase in the surface expression of CD62p (P-selectin) and CD63 (p<0.05), and also in the binding of platelets to monocytes (p<0.05), neutrophilic granulocytes (p<0.05) and to CD3+ cells (initially to a low degree; p<0.05). Platelet binding to CD19+ cells did not change significantly. CONCLUSION: This study demonstrates that platelets become activated during apheresis and that following this process, interaction with monocytes and neutrophilic granulocytes occurs.
BACKGROUND AND OBJECTIVES: In this study we investigated whether platelet activation during apheresis results in the binding of platelets to white blood cells. MATERIAL AND METHODS: Analysis of platelet-leukocyte interaction was performed using multiparameter, three-color flow cytometry. RESULTS: Over the duration of the procedure, there was an increase in the surface expression of CD62p (P-selectin) and CD63 (p<0.05), and also in the binding of platelets to monocytes (p<0.05), neutrophilic granulocytes (p<0.05) and to CD3+ cells (initially to a low degree; p<0.05). Platelet binding to CD19+ cells did not change significantly. CONCLUSION: This study demonstrates that platelets become activated during apheresis and that following this process, interaction with monocytes and neutrophilic granulocytes occurs.