PURPOSE: Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts. MATERIALS AND METHODS: A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was estimated by counting the number of myosin heavy chain (MHC)-positive multinucleated myotubes. Osteoblast differentiation was analysed by measuring alkaline phosphatase (ALP) activity and osteocalcin production in C2C12 cells treated with recombinant human bone morphogenetic protein-2 (rhBMP-2). RESULTS: Myotube formation in C2C12 cells was inhibited by 2 and 4 Gy irradiation, but recovered to non-irradiated control levels following further culture. In irradiated cultures, the number of MHC-positive mononucleated cells increased significantly. Irradiation did not, however, alter the induction of osteoblastic phenotypes induced by rhBMP-2 in C2C12. Irradiation also did not affect the expression of MyoD mRNA and myogenin mRNA in myogenic differentiation and induction of osteocalcin mRNA in osteoblastic differentiation. CONCLUSIONS: During differentiation of C2C12 cells, myotube formation was delayed after irradiation, while induction of osteoblasts with BMP-2 was unaffected.
PURPOSE: Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts. MATERIALS AND METHODS: A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was estimated by counting the number of myosin heavy chain (MHC)-positive multinucleated myotubes. Osteoblast differentiation was analysed by measuring alkaline phosphatase (ALP) activity and osteocalcin production in C2C12 cells treated with recombinant humanbone morphogenetic protein-2 (rhBMP-2). RESULTS: Myotube formation in C2C12 cells was inhibited by 2 and 4 Gy irradiation, but recovered to non-irradiated control levels following further culture. In irradiated cultures, the number of MHC-positive mononucleated cells increased significantly. Irradiation did not, however, alter the induction of osteoblastic phenotypes induced by rhBMP-2 in C2C12. Irradiation also did not affect the expression of MyoD mRNA and myogenin mRNA in myogenic differentiation and induction of osteocalcin mRNA in osteoblastic differentiation. CONCLUSIONS: During differentiation of C2C12 cells, myotube formation was delayed after irradiation, while induction of osteoblasts with BMP-2 was unaffected.
Authors: Oneida A Arosarena; Fabiola E Del Carpio-Cano; Raul A Dela Cadena; Mario C Rico; Emeka Nwodim; Fayez F Safadi Journal: J Cell Physiol Date: 2011-11 Impact factor: 6.384
Authors: John Patrick Connors; Patrick Garvin; Jacob Silver; Adam Lindsay; Olga Solovyova Journal: Arch Orthop Trauma Surg Date: 2022-08-19 Impact factor: 2.928
Authors: Brandon G Santoni; A Simon Turner; Donna L Wheeler; Richard W Nicholas; Tom J Anchordoquy; Nicole Ehrhart Journal: Clin Orthop Relat Res Date: 2008-05-28 Impact factor: 4.176
Authors: Wei Xu; Lan Xu; Ming Chen; Yong Tao Mao; Zong Gang Xie; Shi Liang Wu; Qi Rong Dong Journal: BMC Musculoskelet Disord Date: 2012-06-08 Impact factor: 2.362