Arachidonic acid release and prostaglandin E2 synthesis as irritant index of surfactants in 3T6 fibroblast cultures.

Surface-active agents are components of many drugs and cosmetics. [3H]arachidonic acid ([3H]AA) is rapidly incorporated into cell membrane phospholipids. As a result of membrane disintegration or enzymatic catalysis, it is released to the medium and [3H]AA metabolites can be measured by the scintillation technique. In order to validate the use of this test to predict the irritant and toxic effects of surfactants in vitro, various surfactants were examined using this methodology and the neutral red test. Benzalkonium chloride and sodium dodecylsulfate were more irritant and cytotoxic than amphoteric and non-ionic surfactants such as cocoamidopropylbetaine and Tween 80, respectively. The rank order of the toxicity/irritancy of these agents was similar to that given by other tests in vivo and in vitro. Moreover, our results confirm that the measurement of [3H]AA release in 3T6 fibroblast cultures is a sensitive assay of membrane damage.