beta-endorphin and gonadotropin-releasing hormone in the forebrain and pituitary of the female catfish, Clarias batrachus: double-immunolabeling study.

The role of beta-endorphin in modulating the gonadotropic action of gonadotropin-releasing hormone (GnRH) is well established in mammals. Although the information from teleosts also suggests that endogenous opioids modulate GnRH secretion and influence gonadotropic hormone release, the anatomical substrate in which opiate peptides and GnRH may interact has not been studied. Herein we describe the mammalian GnRH- and beta-endorphin-like immunoreactivities in the olfactory system, forebrain, and pituitary of the teleost, Clarias batrachus, using the double immunocytochemical method. While several olfactory receptor neurons showed beta-endorphin- or GnRH-like immunoreactivity, some neurons with dual immunoreactivities were also seen. GnRH- and/or beta-endorphin-like immunolabeled fascicles were seen in the olfactory nerves as they run caudally to the olfactory bulb and spread in the periphery. Several fascicles branch profusely to form tufts organized as spherical neuropils in the glomerular layer. Frequently, the innervation of the glomeruli showed a distinct pattern. While the fascicles on the medial side showed a predominance of beta-endorphin-like fibers, the majority of the fascicles on the lateral side of the bulb showed dual immunoreactivities. Several GnRH- and beta-endorphin-like immunoreactive fibers were seen in the medial olfactory tract as it extends through the telencephalon in the area ventralis telencephali/pars supracommissuralis; individual fibers with dual staining were also seen. The nucleus lateralis tuberis showed beta-endorphin- as well as GnRH-like immunoreactive neurons. While GnRH-containing cells were seen in the proximal pars distalis and pars intermedia, beta-endorphin-like cells were located throughout the pituitary; some cells in the pars intermedia showed dual immunoreactivity. The high degree of overlapping suggests the possibility of profound interplay between GnRH- and beta-endorphin-like immunoreactive systems at different levels of the neuraxis. Copyright 2000 Academic Press.