| Literature DB >> 10747246 |
J McKerrow1, S Vagg, T McKinney, E M Seviour, A M Maszenan, P Brooks, R J Seviour.
Abstract
A simple and sensitive method for separating and detecting the LL, DD and meso diastereomers of the dibasic amino acid diaminopimelic acid (DAP) in the peptidoglycan of Gram-positive bacteria is described. This method is based on reverse phase HPLC separation of chiral derivatives of DAP followed by fluorescence detection of the o-phthaldehyde derivatives. Its application to the analyses of cell walls of several Gram-positive bacteria is described, where 10 mg or less of dry cells is required.Entities:
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Year: 2000 PMID： 10747246 DOI： 10.1046/j.1472-765x.2000.00675.x
Source DB: PubMed Journal: Lett Appl Microbiol ISSN： 0266-8254 Impact factor: 2.858